Downregulation of PHYTOCHROME-INTERACTING FACTOR 4 Influences Plant Development and Fruit Production

Author(s)

Rosado, Daniele ^[1] ; Trench, Bruna ^[1] ; Bianchetti, Ricardo ^[1] ; Zuccarelli, Rafael ^[1] ; Rodrigues Alves, Frederico Rocha ^[1] ; Purgatto, Eduardo ^[2] ; Segal Floh, Eny Iochevet ^[1] ; Silveira Nogueira, Fabio Tebaldi ^[3] ; Freschi, Luciano ^[1] ; Rossi, Magdalena ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biociencias, Dept Bot, BR-05508090 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP - Brazil

• Document type: Journal article
• Source: Plant Physiology; v. 181, n. 3, p. 1360-1370, NOV 2019.
• Web of Science Citations: 1

Abstract

Plant development is highly dependent on the ability to perceive and cope with environmental changes. In this context, PIF proteins are key players in the cellular hub controlling responses to fluctuating light and temperature conditions. Reports in various plant species show that manipulation of the PIF4 level affects important agronomical traits. In tomato (Solanum lycopersicum), SlPIF1a and SlPIF3 regulate fruit nutraceutical composition. However, the wider role of this protein family, and the potential of their manipulation for the improvement of other traits, has not been explored. Here we report the effects of constitutive silencing of tomato SlPIF4 on whole-plant physiology and development. Ripening anticipation and higher carotenoid levels observed in SlPIF4-silenced fruits revealed a redundant role of SlPIF4 in the accumulation of nutraceutical compounds. Furthermore, silencing triggered a significant reduction in plant size, flowering, fruit yield, and fruit size. This phenotype was most likely caused by reduced auxin levels and altered carbon partitioning. Impaired thermomorphogenesis and delayed leaf senescence were also observed in silenced plants, highlighting the functional conservation of PIF4 homologs in angiosperms. Overall, this work improves our understanding of the role of PIF proteins-and light signaling-in metabolic and developmental processes that affect yield and composition of fleshy fruits. (AU)

• FAPESP's process: 17/24354-7 - Effects of phytochrome-dependent temperature perception in tomato fruit metabolism through epigenetic regulation
• Grantee: Ricardo Ernesto Bianchetti
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/14658-3 - Functional characterization of PIF proteins (Phytochrome-Interacting Factors) in tomato, Solanum lycopersicum
• Grantee: Daniele Silva Pereira Rosado
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 16/16764-8 - Phenotypic characterization of tomato plants (Solanum lycopersicum) knockdown for phytochrome interactions proteins (PIF1b and PIF4)
• Grantee: Bruna Vasconcellos Trench Fonseca
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 16/04924-0 - Manipulating phytochrome levels in tomato fruits (Solanum lycopersicum L.) and its impacts on plastid development, physiology and nutritional composition
• Grantee: Frederico Rocha Rodrigues Alves
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/02033-1 - Manipulation of nitric oxide levels and its impacts on tomato fruit physiology, hormonal balance and nutritional quality
• Grantee: Rafael Zuccarelli
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/01128-9 - Light and hormonal regulation of nutritional quality in Solanum lycopersicum
• Grantee: Maria Magdalena Rossi
• Support Opportunities: Research Projects - Thematic Grants