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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Blood plasma high abundant protein depletion unintentionally carries over 100 proteins

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Author(s):
Silva-Costa, Licia C. [1] ; Garcia-Rosa, Sheila [1] ; Smith, Bradley J. [1] ; Baldasso, Paulo A. [1] ; Steiner, Johann [2] ; Martins-de-Souza, Daniel [3, 1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Lab Neuroprote, Campinas, SP - Brazil
[2] Univ Magdeburg, Dept Psychiat, Magdeburg - Germany
[3] Conselho Nacl Desenvolvimento Cient & Tecnol, Inst Nacl Biomarcadores Neuropsiquiatria INBION, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: SEPARATION SCIENCE PLUS; OCT 2019.
Web of Science Citations: 0
Abstract

There is a constant interest in blood-based protein biomarkers, which can help to improve diagnosis and treatment outcomes of multifactorial human pathologies. In this regard, proteomic studies usually employ plasma immunoaffinity fractionation to deplete the most abundant plasma proteins, due to the high dynamic concentration range of proteins. The depletion of high abundant proteins allows to obtain less abundant and, oftentimes, more interesting proteins. However, the removal of the fraction of the high abundant plasma proteins - the depletome - may co-elute many unintended proteins due to protein-protein interactions. Little data is available about the depletome and potential protein biomarkers may be lost during this process. To visualize and characterize these proteins, we analyzed the depletome of 20 plasma samples by shotgun mass spectrometry-based proteomics. Thus, using immunoaffinity depletion followed by 2-D liquid chromatography coupled to an ion mobility-enhanced mass spectrometer, our analysis identified that over 100 proteins are co-eluting with the high abundant fraction. These proteins play roles in several biological processes, such as receptor-mediated endocytosis, complement activation, and regulation of immune response. This study supports that investigating the depletome is important in the quest for biomarkers. (AU)

FAPESP's process: 13/08711-3 - Developing a predictive test for a successful medication response and understanding the molecular bases of schizophrenia through proteomics
Grantee:Daniel Martins-de-Souza
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 17/25588-1 - From the basic understanding to clinical biomarkers to schizophrenia: a neuroproteomics-centered multidisciplinary study
Grantee:Daniel Martins-de-Souza
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/07948-8 - Relationship between a Myelin-Associated Phosphodiesterase CNP and Schizophrenia
Grantee:Bradley Joseph Smith
Support type: Scholarships in Brazil - Master
FAPESP's process: 18/03422-7 - Identification of blood-based biomarkers for late-life depression through proteomics
Grantee:Licia Carla da Silva Costa
Support type: Scholarships in Brazil - Master
FAPESP's process: 14/10068-4 - Multi-User Equipment approved in grant 13/08711-3: mass spectrometer waters SYNAPT G2-Si HDMS + nanoACQUITY UPLC
Grantee:Daniel Martins-de-Souza
Support type: Multi-user Equipment Program
FAPESP's process: 19/05747-3 - Maintaining and maturing oligodendrocyte cell line
Grantee:Bradley Joseph Smith
Support type: Scholarships in Brazil - Technical Training Program - Technical Training