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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

3D Printed Cartilage-Like Tissue Constructs with Spatially Controlled Mechanical Properties

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de Melo, Bruna A. G. [1, 2] ; Jodat, Yasamin A. [2, 3] ; Mehrotra, Shreya [2, 4] ; Calabrese, Michelle A. [5] ; Kamperman, Tom [6] ; Mandal, Biman B. [4] ; Santana, Maria H. A. [1] ; Alsberg, Eben [7, 8] ; Leijten, Jeroen [6] ; Shin, Su Ryon [2]
Total Authors: 10
[1] Univ Estadual Campinas, Sch Chem Engn, Dept Engn Mat & Bioproc, BR-13083852 Campinas, SP - Brazil
[2] Harvard Med Sch, Brigham & Womens Hosp, Dept Med, Div Engn Med, Cambridge, MA 02139 - USA
[3] Stevens Inst Technol, Dept Mech Engn, Hoboken, NJ 07030 - USA
[4] Indian Inst Technol Guwahati, Dept Biosci & Bioengn, Gauhati 781039, Assam - India
[5] Univ Minnesota, Dept Chem Engn & Mat Sci, 421 Washington Ave SE, Minneapolis, MN 55455 - USA
[6] Univ Twente, Dept Dev BioEngn, NL-7522 NB Enschede, Overijssel - Netherlands
[7] Univ Illinois, Dept Bioengn, Chicago, IL 60607 - USA
[8] Univ Illinois, Dept Orthopaed, Chicago, IL 60607 - USA
Total Affiliations: 8
Document type: Journal article
Web of Science Citations: 0

Developing biomimetic cartilaginous tissues that support locomotion while maintaining chondrogenic behavior is a major challenge in the tissue engineering field. Specifically, while locomotive forces demand tissues with strong mechanical properties, chondrogenesis requires a soft microenvironment. To address this challenge, 3D cartilage-like tissue is fabricated using two biomaterials with different mechanical properties: a hard biomaterial to reflect the macromechanical properties of native cartilage, and a soft biomaterial to create a chondrogenic microenvironment. To this end, a bath composed of an interpenetrating polymer network (IPN) of polyethylene glycol (PEG) and alginate hydrogel (MPa order compressive modulus) is developed as an extracellular matrix (ECM) with self-healing properties. Within this bath supplemented with thrombin, human mesenchymal stem cell (hMSC) spheroids embedded in fibrinogen are 3D bioprinted, creating a soft microenvironment composed of fibrin (kPa order compressive modulus) that simulate cartilage's pericellular matrix and allow a fast diffusion of nutrients. The bioprinted hMSC spheroids present high viability and chondrogenic-like behavior without adversely affecting the macromechanical properties of the tissue. Therefore, the ability to locally bioprint a soft and cell stimulating biomaterial inside of a mechanically robust hydrogel is demonstrated, thereby uncoupling the micro- and macromechanical properties of the 3D printed tissues such as cartilage. (AU)

FAPESP's process: 17/02913-4 - Microfluidics for 3D h-ADMCs culture in hyaluronic acid free or structured in sponges: chemotaxis and chemokinesis in response to growth factors gradients from L-PRP
Grantee:Bruna Alice Gomes de Melo
Support type: Scholarships abroad - Research Internship - Doctorate