de Melo, Bruna A. G.
Jodat, Yasamin A.
Calabrese, Michelle A.
Mandal, Biman B.
Santana, Maria H. A.
Shin, Su Ryon
Total Authors: 10
 Univ Estadual Campinas, Sch Chem Engn, Dept Engn Mat & Bioproc, BR-13083852 Campinas, SP - Brazil
 Harvard Med Sch, Brigham & Womens Hosp, Dept Med, Div Engn Med, Cambridge, MA 02139 - USA
 Indian Inst Technol Guwahati, Dept Biosci & Bioengn, Gauhati 781039, Assam - India
 Univ Minnesota, Dept Chem Engn & Mat Sci, 421 Washington Ave SE, Minneapolis, MN 55455 - USA
 Univ Twente, Dept Dev BioEngn, NL-7522 NB Enschede, Overijssel - Netherlands
 Univ Illinois, Dept Bioengn, Chicago, IL 60607 - USA
 Univ Illinois, Dept Orthopaed, Chicago, IL 60607 - USA
Total Affiliations: 8
ADVANCED FUNCTIONAL MATERIALS;
Web of Science Citations:
Developing biomimetic cartilaginous tissues that support locomotion while maintaining chondrogenic behavior is a major challenge in the tissue engineering field. Specifically, while locomotive forces demand tissues with strong mechanical properties, chondrogenesis requires a soft microenvironment. To address this challenge, 3D cartilage-like tissue is fabricated using two biomaterials with different mechanical properties: a hard biomaterial to reflect the macromechanical properties of native cartilage, and a soft biomaterial to create a chondrogenic microenvironment. To this end, a bath composed of an interpenetrating polymer network (IPN) of polyethylene glycol (PEG) and alginate hydrogel (MPa order compressive modulus) is developed as an extracellular matrix (ECM) with self-healing properties. Within this bath supplemented with thrombin, human mesenchymal stem cell (hMSC) spheroids embedded in fibrinogen are 3D bioprinted, creating a soft microenvironment composed of fibrin (kPa order compressive modulus) that simulate cartilage's pericellular matrix and allow a fast diffusion of nutrients. The bioprinted hMSC spheroids present high viability and chondrogenic-like behavior without adversely affecting the macromechanical properties of the tissue. Therefore, the ability to locally bioprint a soft and cell stimulating biomaterial inside of a mechanically robust hydrogel is demonstrated, thereby uncoupling the micro- and macromechanical properties of the 3D printed tissues such as cartilage. (AU)