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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

IgG from atopic dermatitis patients induces non-atopic infant thymic invariant natural killer T (iNKT) cells to produce IL-4, IL-17, and IL-10

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Author(s):
Santos, Ludimila S. [1, 2] ; Sgnotto, Fabio da Ressureicao [3] ; Sousa, Thamires R. [2] ; Orfali, Raquel L. [2] ; Aoki, Valeria [2] ; da Silva Duarte, Alberto Jose [4, 2] ; Victor, Jefferson R. [1, 2]
Total Authors: 7
Affiliation:
[1] Laureate Int Univ, FMU, Div Environm Hlth, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, Div Clin Dermatol, Lab Med Invest LIM 56, Ave Dr En eas de Carvalho Aguiar 500, 3rd Floor, BR-05403000 Sao Paulo - Brazil
[3] Univ Sao Paulo, Div Hematol, Sch Med, Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Med, Div Pathol, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF DERMATOLOGY; OCT 2019.
Web of Science Citations: 0
Abstract

Background Atopic dermatitis (AD) pathogenesis still needs to be elucidated, but invariant natural killer T (iNKT) cell involvement was already described by several groups. Our group has demonstrated that IgG antibodies purified from AD patients can modulate cytokine production by thymic T cells. Here we aimed to investigate if IgG from AD patients can modulate infant non-atopic thymic iNKT cells cytokine production in order to collaborate with the elucidation of AD development in infancy. Methods Thymic tissues were obtained from children from non-atopic mothers, and IgG was purified from AD patients diagnosed as moderate or severe and, as controls, from subjects clinically classified as non-atopic individuals. PBMCs from non-atopic individuals were also used in this study. Results Our results demonstrated that IgG from AD patients could induce non-atopic children thymic iNKT cells to produce higher levels of intracellular IL-4, IL-10, and IL-17 when compared to all control conditions. No effect was observed in non-atopic adults peripheral iNKT. We also observed that IgG from AD patients induces an increase in the expression of CD4 and Ror gamma t transcription factor in non-atopic children thymic iNKT cells compared to the condition of all controls. Conclusions These observations suggest that IgG from AD patients can induce a cytokine profile by thymic iNKT cells from non-atopic infants compatible with the observations in AD development, which can collaborate with the elucidation of AD pathogenesis. (AU)

FAPESP's process: 18/05181-7 - Translational evaluation of IgG effect upon the maturation of offspring thymus derived TCD4, TCD8, nTreg, gammadeltaT and e B cells.
Grantee:Alberto José da Silva Duarte
Support type: Regular Research Grants