Comparison between micro-computed tomography and transverse microradiography of sound dentine treated with fluorides and demineralized by microcosm biofilm

Author(s)

Soares dos Santos, Daiana M. ^[1] ; Braga, Aline S. ^[1] ; Rizk, Marta ^[2] ; Wiegand, Annette ^[2] ; Magalhaes, Ana C. ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Al Dr Octavio Pinheiro Brisolla 9-75, Cidade Univ, BR-17012901 Bauru, SP - Brazil

^[2] Univ Med Ctr Gottingen, Dept Prevent Dent Periodontol & Cariol, Gottingen - Germany

• Document type: Journal article
• Source: European Journal of Oral Sciences; OCT 2019.
• Web of Science Citations: 0

Abstract

The study aimed to apply micro-computed tomography (micro-CT) and transverse microradiography (TMR) to measure dentine demineralization and to test the preventive effect of titanium tetrafluoride (TiF4) under microcosm biofilm. Sound dentine specimens from bovine root were treated for 6 h with: (i) 4.0% titanium tetrafluoride (TiF4) varnish {[}pH 1.0, 2.45% fluoride (F-); (ii) 5.42% sodium fluoride (NaF) varnish (pH 5.0, 2.45% F); (iii) 2% chlorhexidine (CHX) gel (pH 7.0); (iv) placebo varnish (pH 5.0); or (v) no agent (untreated). Dentine specimens were then exposed to human saliva mixed with McBain saliva for 8 h. Thereafter, McBain saliva containing 0.2% sucrose was applied daily, for 5 d, onto dentine specimens to stimulate formation of microcosm biofilm. Although a high correlation was found between the results of both methods regarding integrated mineral loss, the results of the methods did not show good agreement in Bland-Altman plots, with significant biases in calculations of lesion depth. Fluoride varnishes were able to reduce dentine demineralization (P < 0.05), while CHX failed to do so. Fluorides are still the best option to reduce dentine demineralization. Micro-CT may be used to measure dentine mineral loss, but not the lesion depth, for which TMR is superior. (AU)

• FAPESP's process: 17/13386-5 - Effect of 4% titanium tetrafluoride varnish on the prevention of dentin demineralization under a cariogenic microcosm biofilm model - Micro-CT and TMR analysis
• Grantee: Daiana Moreli Soares dos Santos
• Support type: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 17/00556-0 - Evaluation of the antimicrobial effect of alternative and commercial agents on a microcosm biofilm and on prevention of dental demineralization
• Grantee: Ana Carolina Magalhães
• Support type: Regular Research Grants