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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of Angiotensin I-Converting Enzyme-Inhibitory and Anticoagulant Peptides from Enzymatic Hydrolysates of Chicken Combs and Wattles

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Alencar Bezerra, Taliana Kenia [1] ; Jocelino Gomes de Lacerda, Jose Thalles [2] ; Salu, Bruno Ramos [3] ; Vilela Oliva, Maria Luiza [3] ; Juliano, Maria Aparecida [2] ; Pacheco, Maria Teresa Bertoldo [4] ; Madruga, Marta Suely [1]
Total Authors: 7
[1] Univ Fed Paraiba, Technol Ctr, Dept Food Engn, Postgrad Program Food Sci & Technol, BR-58051900 Joao Pessoa, Paraiba - Brazil
[2] Univ Fed Sao Paulo, Dept Biophys, Sao Paulo, SP - Brazil
[3] Univ Fed Sao Paulo, Dept Biochem, Sao Paulo, SP - Brazil
[4] Ctr Food Sci & Qual, Food Technol Inst ITAL, Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF MEDICINAL FOOD; v. 22, n. 12 DEC 2019.
Web of Science Citations: 0

Peptides from protein hydrolysate of a mixture of chicken combs and wattles (CCWs) were obtained through enzymatic hydrolysis, and their anticoagulant and inhibitory effects on angiotensin I-converting enzyme (ACE) were investigated. The protein hydrolysate exhibited anticoagulant capacity by the intrinsic pathway (activated partial thromboplastin time) and potent ACE-inhibitory activity. The peptides were sequenced by LC-MS to identify those with higher inhibitory potential. From the pool of sequenced peptides, the following three peptides were selected and synthesized based on their low molecular weight and the presence of amino acids with ACE-inhibitory potential at the C-terminus: peptide I (APGLPGPR), peptide II (Piro-GPPGPT), and peptide III (FPGPPGP). Peptide III (FPGPPGP) showed the highest ACE-inhibitory capacity among the peptides selected. In conclusion, a peptide (FPGPPGP) of unknown sequence was identified as having potent ACE-inhibitory capacity. This peptide originated from unconventional hydrolysates from poultry slaughter waste, including combs and wattles. (AU)

FAPESP's process: 18/13588-0 - Multi - user equipment approved in grant 2012/50191-4: mass spectrometer impact II - Bruker
Grantee:Maria Aparecida Juliano
Support type: Multi-user Equipment Program
FAPESP's process: 12/50191-4 - Synthesis, kinetic studies and applications of substrates and inhibitors for proteolytic enzymes
Grantee:Maria Aparecida Juliano
Support type: Research Projects - Thematic Grants