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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Graphene oxide-based nanomaterial interaction with human breast cancer cells

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Author(s):
Ribeiro, Beatriz F. M. [1] ; Souza, Marcelo M. [1] ; Fernandes, Daniela S. [2, 3] ; do Carmo, Devaney R. [2] ; Machado-Santelli, Glaucia M. [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Sao Paulo, SP - Brazil
[2] Univ Estadual Paulista, Dept Phys & Chem, Sao Paulo, SP - Brazil
[3] Fac Philosophy Sci & Arts Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Dept Chem, Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Biomedical Materials Research Part A; v. 108, n. 4 DEC 2019.
Web of Science Citations: 0
Abstract

Graphene and graphene-based nanomaterials have great potential for various biomedical applications due to their unique physicochemical properties. However, how graphene-based nanomaterials interact with biological systems has not been thoroughly studied. This study shows that 24, 48, and 72 hr exposure of 2.4 mu g/cm(2) of graphene oxide (GOX) and GOX modified with DAB-AM-16 and PAMAM dendrimers (GOXD and GOXP, respectively) did not exhibit toxicity to MCF-7 cells. However, higher graphene concentrations, such as 24 and 48 mu g/cm(2), induced low cytotoxic effects. The GOX, GOXD, and GOXP particles have a strong affinity with the cellular membrane. Cells that internalized the nanomaterials presented morphological alterations and modifications in the organization of microfilaments and microtubules compared with control cells. Then, cells were treated with 24 mu g/cm(2) of GOX, GOXD or GOXP for 24 hr and recovered for an additional period of 24 hr in normal medium. Nanoparticles remained in the cytoplasm of some cells, apparently with no effect on cellular morphology, being consistent with the data found in the cell proliferation experiment, which showed that the cells remained alive up to 72 hr. (AU)

FAPESP's process: 18/22882-9 - Interiorization of graphene oxide by tumor cells in culture: evaluation of mechanisms and effects
Grantee:Beatriz Fumelli Monti Ribeiro
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 17/16893-5 - Study of the graphene oxide effects on the microfilament and microtubule network in MCF7 cell line
Grantee:Beatriz Fumelli Monti Ribeiro
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 15/20397-8 - Surface modification of graphene oxide with a dendrimer: A study of hybrid nanostructured materials for chemical and biological applications
Grantee:Devaney Ribeiro Do Carmo
Support type: Regular Research Grants