In vitro bioactivity and biological assays of porous membranes of the poly(lactic acid) containing calcium silicate fibers

Author(s)

Waltrick Brito Siqueira, Idalia Aparecida ^[1] ; Amaral, Suelen Simoes ^[2] ; de Moura, Nayara Koba ^[1] ; Barros Machado, Joao Paulo ^[3] ; Backes, Eduardo Henrique ^[4] ; Passador, Fabio Roberto ^[1] ; Afonso Camargo, Samira Esteves ^[5] ; Reis de Vasconcellos, Luana Marotta ^[2] ; Triches, Eliandra Sousa ^[1]

Affiliation

^[1] Fed Univ Sao Paulo UNIFESP, 330 Talim St, BR-12231280 Sao Jose Dos Campos, SP - Brazil

^[2] State Univ Sao Paulo UNESP, UNESP Univ Estadual Paulista, Inst Sci & Technol, 777 Av Eng Francisco Jose Longo, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[3] Natl Inst Space Res INPE, 1758 Astronautas Ave, BR-12227010 Sao Jose Dos Campos, SP - Brazil

^[4] Univ Fed Sao Carlos, 235 Km Washington Luis, BR-13565905 Sao Carlos, SP - Brazil

^[5] Univ Florida UFL, Coll Dent, Dept Restorat Dent Sci, Gainesville, FL 32611 - USA

• Document type: Journal article
• Source: POLYMER BULLETIN; v. 77, n. 10 NOV 2019.
• Web of Science Citations: 0

Abstract

A new approach in dentistry is based on the development of resorbable polymeric membranes for guided bone regeneration. The porous membrane surface promotes better cell adhesion and proliferation. Poly(lactic acid) (PLA) is one of most widely used polymers for several biological applications, with the advantage of presenting hydrolysis degradation and bioresorption of its products. In this work, PLA porous polymeric membranes containing calcium silicate (CaSiO3) fibers were prepared by controlled humidity technique. The porous membranes were characterized by scanning electron microscopy, X-ray diffraction, differential scanning calorimetry, bioactivity in simulated body fluid and biological studies in vitro. The incorporation of the CaSiO3 fibers into the polymeric matrix increased the pore size (1.5-34.9 mu m). In the bioactivity assay, PLA/CASiO(3) membranes induced the formation of a hydroxyapatite layer on the porous membrane surface. Also, in vitro biologic assays showed that the porous membranes provided suitable environment for cell attachment and proliferation. (AU)

• FAPESP's process: 15/24659-7 - SYNTHESIS, CHARACTERIZATION AND BIOCOMPATIBILITY EVALUATION OF BIOCERAMIC SCAFFOLDS PREPARED BY FREEZE CASTING METHOD
• Grantee: Eliandra de Sousa Trichês
• Support Opportunities: Regular Research Grants