Effects of surface pre-reacted glass-ionomer (S-PRG) eluate on Candida spp.: antifungal activity, anti-biofilm properties, and protective effects on Galleria mellonella against C. albicans infection

Author(s)

Rossoni, Rodnei Dennis ^[1] ; de Barros, Patricia Pimentel ^[1] ; das Chagas Lopes, Lucas Alexandre ^[1] ; Ribeiro, Felipe Camargo ^[1] ; Nakatsuka, Toshiyuki ^[2] ; Kasaba, Hideto ^[2] ; Junqueira, Juliana Campos ^[1]

Affiliation

^[1] UNESP Univ Estadual Paulista, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Jose Dos Campos - Brazil

^[2] SHOFU Inc, Dept Res, Kyoto - Japan

• Document type: Journal article
• Source: BIOFOULING; NOV 2019.
• Web of Science Citations: 1

Abstract

Surface pre-reacted glass-ionomer (S-PRG) is a bioactive filler produced by PRG technology, which is applied to various dental materials. The inhibitory effects of S-PRG eluate against Candida, the most common fungal oral pathogen, were investigated. Minimum inhibitory concentrations (MIC) and anti-biofilm activities were tested against Candida albicans, Candida glabrata, Candida krusei, and Candida tropicalis. For the in vivo study, Galleria mellonella was used as a model to evaluate the effects of S-PRG on toxicity, hemocyte counts and candidiasis. The MIC of S-PRG ranged from 5 to 40% (v/v). S-PRG eluate exhibited anti-biofilm activity for all the Candida species tested. Furthermore, injection of S-PRG eluate into G. mellonella was not toxic to the larvae and protected G. mellonella against experimental candidiasis. In addition, S-PRG eluate inhibited biofilm formation by C. albicans, C. glabrata, C. krusei, and C. tropicalis and exerted protective effects on G. mellonella against experimental candidiasis in vivo. (AU)

• FAPESP's process: 19/05664-0 - Exploring the caffeic acid (CA), caffeic acid phenethyl ester (CAPE) and artepillin c (ARTC) as new strategies to eliminate persisters cells of Candida albicans.
• Grantee: Patricia Pimentel de Barros
• Support type: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 17/02652-6 - Evaluation of the immunomodulatory and antifungal effects of caffeic acid (CA), caffeic acid phenethyl ester (CAPE) and artepillin C (ARC) on C. albicans and development of candidiasis in in vivo model hosts
• Grantee: Patricia Pimentel de Barros
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 17/19219-3 - Identification of bioactive substances produced by Lactobacillus paracasei 28.4 for the control of oral candidiasis
• Grantee: Rodnei Dennis Rossoni
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 18/21239-5 - Galleria mellonella in antimicrobial drug discovery: bioactive substances extracted from probiotic against Candida albicans persisters
• Grantee: Rodnei Dennis Rossoni
• Support type: Scholarships abroad - Research Internship - Post-doctor