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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil

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Author(s):
Vilela, Felipe Pinheiro [1] ; Rodrigues, Dalia dos Prazeres [2] ; Costa, Renata Garcia [2] ; Tiba Casas, Monique Ribeiro [3] ; Falcao, Juliana Pfrimer [1] ; Campioni, Fabio [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Av Caf S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Fundacao Oswaldo Cruz, FIOCRUZ, Av Brasil 4365, BR-21040900 Rio De Janeiro, RJ - Brazil
[3] Adolfo Lutz Inst, Ctr Bacteriol, Av Dr Arnaldo 351, BR-01255000 Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Brazilian Journal of Microbiology; v. 51, n. 2 NOV 2019.
Web of Science Citations: 1
Abstract

Salmonella Dublin is a strongly adapted serovar that causes enteritis and/or systemic disease with high rates of mortality in cattle and occasionally infects humans. Despite the importance of this serovar, there is a lack of studies in Brazil. The aim of this study was to characterize the genetic diversity of 112 S. Dublin strains isolated from humans and animals in Brazil by CRISPR and CRISPR-MVLST and the relatedness among strains by MLST. In addition, the frequency of some important virulence genes was verified. The strains studied belonged to nine different sequence types, being all of them single- or double-locus variants of the ST10. CRISPR discriminated the strains into 69 subtypes with a similarity >= 84.4% and CRISPR-MVLST into 72 subtypes with a similarity >= 84.7%. The virulence genes ratB, lpfA, mgtC, avrA, sopB, sopE2, sifA, sseA, ssrA, csgA, fliC, and sinH were found in all the strains studied, while spvB, spvC, sodCl, rpoS, sipA, sipD, invA, and hilA were detected in >= 93.7% of the strains. In conclusion, the high similarity among the strains reinforces the clonal nature of the strains of this serovar that may have descended from a common ancestor that little differed over 33 years in Brazil. CRISPR and CRISPR-MVLST showed to be good alternatives to type S. Dublin strains. MLST suggested that S. Dublin strains from Brazil were phylogenetically related to strains from other parts of the globe. Moreover, the high frequency of virulence genes among the strains studied reinforces the capacity of S. Dublin to cause invasive diseases. (AU)

FAPESP's process: 17/05756-7 - Cell invasion, macrophage survival analysis and expression of the genes st313-td, sopE2 and fliC in Salmonella Dublin strains isolated in Brazil
Grantee:Felipe Pinheiro Vilela
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 19/06947-6 - Molecular characterization of the genotypic diversity, resistance profile and pathogenic potential of Salmonella Infantis strains isolated from multiple sources in Brazil
Grantee:Felipe Pinheiro Vilela
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 13/25191-3 - Phenotypical analysis and transcriptome and genome sequencing in Salmonella Enteritidis strains isolated in the pre and post-pandemic period in Brazil
Grantee:Fábio Campioni
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/24716-3 - Sequencing of the Genome, transcriptoma and phenotypic analysis of Campylobacter coli strains isolated from different sources in Brazil
Grantee:Juliana Pfrimer Falcão
Support Opportunities: Regular Research Grants