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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Gene and protein expression of myosin heavy chain in Nellore cattle comparing growth or meat tenderness traits

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Author(s):
Chardulo, L. A. L. [1] ; Baldassini, W. A. [1] ; Curi, R. A. [1] ; Pereira, G. L. [1] ; Machado Neto, O. R. [1] ; Dal-Pai, M. [2] ; Vechetti-Junior, I. J. [2] ; Malheiros, J. M. [3] ; Enriquez-Valencia, C. E. [4]
Total Authors: 9
Affiliation:
[1] Sao Paulo State Univ UNESP, Coll Vet Med & Anim Sci, BR-18618000 Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Inst Biosci, Botucatu, SP - Brazil
[3] Embrapa Pecuaria Sudeste, Sao Carlos, SP - Brazil
[4] Univ Ciencias Aplicadas & Ambientales UDCA, Bogota - Colombia
Total Affiliations: 4
Document type: Journal article
Source: ANIMAL BIOTECHNOLOGY; v. 32, n. 3 NOV 2019.
Web of Science Citations: 3
Abstract

The objective was to investigate gene and protein expression of myosin heavy chain (MyHC) in Nellore cattle slaughtered at different weights (BW) or degrees of meat tenderness. Ninety animals with initial BW 370 +/- 37 kg, 24 months of age, were slaughtered after 95 days on feed. We evaluated shear force (SF), myofibrillar fragmentation index, ribeye area, backfat thickness, marbling, color, and cooking losses. Subsequently, 24 animals were selected and divided into four contrasting groups, in which light (BW = 504.58 +/- 32.36 kg) versus heavy animals (BW = 604.83 +/- 42.97 kg) and animals with tender (SF = 3.88 +/- 0.57 kg) versus tough meat (SF = 7.95 +/- 1.04 kg) were compared. The MYH7, MYH2 and MYH1 genes were analyzed by real-time PCR. The MyHC isoforms (MyHC-I, MyHC-IIa, and MyHC-IIx) were quantified by SDS-PAGE electrophoresis. We found lower expression of MYH2 and MYH1 genes in heavy compared to light animals and a higher amount of MyHC-I isoform in the tough meat group compared to the tender meat group. Protein expression of MyHC-IIa was higher in the tender meat group. A negative correlation was found of this protein and SF (tenderness), suggesting MyHC-IIa as a biomarker of meat quality. (AU)

FAPESP's process: 18/00981-5 - Identification of regulatory genes for tenderness and lipogenesis by transcriptomic and proteomic approach in beef cattle fed with different diets
Grantee:Welder Angelo Baldassini
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/13021-1 - An metalloproteomics and expression approach of the structural, proteolytic and tissue skeletal muscle regulatory proteins in post-mortem and the relationship with growth and meat tenderness in Nellore animals (Bos indicus).
Grantee:Luis Artur Loyola Chardulo
Support type: Regular Research Grants