Alginate films functionalized with silver sulfadiazine-loaded [Mg-Al] layered double hydroxide as antimicrobial wound dressing

Author(s)

Munhoz, Davi R. ^[1] ; Bernardo, Marcela P. ^[1] ; Malafatti, Joao O. D. ^{[2, 1]} ; Moreira, Francys K. V. ^[3] ; Mattoso, Luiz H. C. ^[1]

Affiliation

^[1] Embrapa Instrumentat, LNNA, 1452 15 Novembro St, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Chem, Rod Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Carlos, Dept Mat Engn, Rod Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: International Journal of Biological Macromolecules; v. 141, p. 504-510, DEC 1 2019.
• Web of Science Citations: 3

Abstract

Alginate (ALG) is an abundant, biocompatible, regenerative, and nontoxic polysaccharide that has potential applications in tissue engineering. Silver sulfadiazine (SDZ) is a topical antibiotic used to control bacterial infection in burns. Aiming to combine the intrinsic alginate characteristics and silver sulfadiazine antimicrobial properties, hydrotalcite ({[}Mg-Al]-LDH) was used as a host matrix to obtain a system efficient in delivering SDZ from alginate films. SDZ was successfully intercalated in {[}Mg-Al]-LDH through structural reconstruction. Different solutions were prepared using sodium alginate at 10 wt%, glycerol at 10 wt% as a plasticizer and {[}Mg-Al]-LDH and {[}Mg-Al]-LDH/SDZ as fillers at 1 wt% and 5 wt%. Films were obtained by continuous casting and further characterized for their microstructural, mechanical, water barrier and antimicrobial properties. Cytotoxicity tests were also performed on fibroblasts cells. The incorporation of {[}Mg-Al]-LDH and {[}Mg-Al]-LDH/SDZ presented neither negative nor positive effects on the mechanical properties and morphology of the alginate films. Moreover, samples containing SDZ exhibited inhibitory activity against S. aureus, E. coli, and S. enterica. The addition of {[}Mg-Al]-LDH/SDZ even at the highest concentration did not afford a very significant cytotoxicity to the alginate-{[}Mg-Al]-LDH/SDZ films. These results describe a suitable approach for preparing innovative active wound dressings integrated to efficient drug delivery. (C) 2019 Published by Elsevier B.V. (AU)

• FAPESP's process: 10/11584-5 - Development of biodegradable polymeric nanocomposites from pectin, starch and layered double hydroxides
• Grantee: Francys Kley Vieira Moreira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/21867-0 - Development of bionanocomposite films based on starch, pectin and layered double hydroxides through continuous process
• Grantee: Francys Kley Vieira Moreira
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 18/07860-9 - Development of nanocomposite polymer filaments with anti-inflammatory properties for 3D printing as bone substituent
• Grantee: Marcela Piassi Bernardo
• Support Opportunities: Scholarships in Brazil - Post-Doctorate