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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Shotgun proteomics of Strongyloides venezuelensis infective third stage larvae: Insights into host-parasite interaction and novel targets for diagnostics

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Author(s):
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Fonseca, Priscilla D. M. [1, 2] ; Corral, Marcelo A. [2] ; Cosenza-Contreras, Miguel [3] ; Meisel, Dirce M. C. L. [2] ; Melo, Gessica B. [1] ; Antunes, Milena M. S. [2] ; Santo, Maria C. E. [2] ; Gryschek, Ronaldo C. B. [1, 2] ; Costa-Cruz, Julia M. [4] ; Castro-Borges, William [3] ; Paula, Fabiana M. [1, 2]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, Fac Med, Inst Med Trop, Sao Paulo - Brazil
[2] Univ Sao Paulo, Lab Invest Med, Fac Med, Sao Paulo - Brazil
[3] Univ Fed Ouro Preto, Inst Ciencias Exatas & Biol, Lab Enzimol & Prote, Ouro Preto, MG - Brazil
[4] Univ Fed Uberlandia, Lab Diagnost Parasitoses, Inst Ciencias Biomed, Uberlandia, MG - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Molecular and Biochemical Parasitology; v. 235, JAN 2020.
Web of Science Citations: 0
Abstract

Strongyloides venezuelensis is an important alternative source of antigen for the serologic diagnosis of human strongyloidiasis. Proteomics techniques applied to the analysis of the protein content of infective third stage larvae (iL3) of S. venezuelensis provide a powerful tool for the discovery of new candidates for immunodiagnosis. This study presents an overview of the protein iL3 S. venezuelensis focusing on the diagnosis of strongyloidiasis. A total of 877 proteins were identified by shotgun proteomics. Many of these proteins are involved in different cellular processes, metabolic as well as structural maintenance. Our results point to a catalog of possible diagnostic targets for human strongyloidiasis and highlight the need for evaluation of uncharacterized proteins, especially the proteins within the CAP domain, transthyretin, and BTPI inhibitor domains, as a repertoire as yet unexplored in the context of strongyloidiasis diagnostic markers. We believe that the protein profile presented in this shotgun analysis extends our understanding of the protein composition within the Strongyloides genus, opening up new perspectives for research on biomarkers that may help with the diagnosis of human strongyloidiasis. Data are available via ProteomeXchange with identifier PXD013703. (AU)

FAPESP's process: 16/06185-0 - Proteomic analysis of evolutionary stages from Strongyloides venezuelensis
Grantee:Fabiana Martins de Paula
Support Opportunities: Regular Research Grants