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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification and distribution of mycosporine-like amino acids in Brazilian cyanobacteria using ultrahigh-performance liquid chromatography with diode array detection coupled to quadrupole time-of-flight mass spectrometry

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Author(s):
Geraldes, Vanessa [1] ; Jacinavicius, Fernanda Rios [1] ; Genuario, Diego Bonaldo [2] ; Pinto, Ernani [3, 1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Fac Pharmaceut Sci, Sao Paulo, SP - Brazil
[2] Embrapa Environm, Lab Environm Microbiol, Jaguariuna, SP - Brazil
[3] Univ Sao Paulo, Ctr Nucl Energy Agr, Piracicaba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: RAPID COMMUNICATIONS IN MASS SPECTROMETRY; FEB 2020.
Web of Science Citations: 1
Abstract

Rationale Mycosporine-like amino acids (MAAs) are UV-absorbing compounds produced by fungi, algae, lichens, and cyanobacteria when exposed to UV radiation. These compounds have photoprotective and antioxidant functions and have been widely studied for possible use in sunscreens and anti-aging products. This study aims to identify MAA-producing cyanobacteria with potential application in cosmetics. Methods A method for the identification of MAAs was developed using ultrahigh-performance liquid chromatography with diode array detection coupled to quadrupole time-of-flight mass spectrometry (UHPLC-DAD/QTOFMS). Chromatographic separation was carried out using a Synergi 4 mu Hydro-RP 80A column (150 x 2,0 mm) at 30 degrees C with 0.1% formic acid aqueous solution + 2 mM ammonium formate and acetonitrile/water (8:2) + 0.1% formic acid as a mobile phase. Results Out of the 69 cyanobacteria studied, 26 strains (37%) synthesized MAAs. Nine different MAAs were identified using UHPLC-DAD/QTOFMS. Iminomycosporines were the major group detected (7 in 9 MAAs). In terms of abundance, the most representative genera for MAA production were heterocyte-forming groups. Oscilatoria sp. CMMA 1600, of homocyte type, produced the greatest diversity of MAAs. Conclusions The UHPLC-DAD/QTOFMS method is a powerful tool for identification and screening of MAAs in cyanobacterial strains as well as in other organisms such as dinoflagellates, macroalgae, and microalgae. The different cyanobacterial genera isolated from diverse Brazilian biomes and environments are prolific sources of MAAs. (AU)

FAPESP's process: 14/26131-7 - Microbiome of Amazon River and its tributaries: biogeography and contribution to N2 cycle
Grantee:Diego Bonaldo Genuário
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/50420-9 - Secondary metabolites from aquatic microrganisms and their impact on seafood and freshwater fish aquaculture
Grantee:Ernani Pinto Junior
Support type: Regular Research Grants