Effect of cell therapy with osteoblasts differentiated from bone marrow or adipose tissue stromal cells on bone repair

Author(s)

Freitas, Gileade P. ^[1] ; Lopes, Helena B. ^[1] ; Souza, Alann T. P. ^[1] ; Oliveira, Paula G. F. P. ^[1] ; Almeida, Adriana L. G. ^[1] ; Coelho, Paulo G. ^{[2, 3]} ; Ferreira, Fernanda U. ^[4] ; Covas, Dimas T. ^{[5, 4]} ; Beloti, Marcio M. ^[6] ; Rosa, Adalberto L. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral & Maxillofacial Surg & Periodontol, Sao Paulo, SP - Brazil

^[2] NYU, Sch Med, Hansjorg Wyss Dept Plast Surg, New York, NY 10016 - USA

^[3] NYU, Coll Dent, Dept Biomat, New York, NY 10010 - USA

^[4] Univ Sao Paulo, Sch Med Ribeirao Preto, Reg Blood Ctr Ribeirao Preto, Ctr Cell Based Res, Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Clin Med, Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Basic & Oral Biol, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: REGENERATIVE MEDICINE; v. 14, n. 12, p. 1107-1119, DEC 2019.
• Web of Science Citations: 0

Abstract

Aim: The aim of this study was to investigate the effect of local injection of osteoblasts differentiated from bone marrow (BM-OB) or adipose tissue (AT-OB) mesenchymal stromal cells on bone tissue formation. Materials \& methods: Defects were created in rat calvaria and injected with BM-OB or AT-OB and phosphate-buffered saline without cells were injected as control. Bone formation was evaluated 4 weeks postinjection. Results: Injection of BM-OB or AT-OB resulted in higher bone formation than that obtained with control. The bone tissue induced by cell injections exhibited similar mechanical properties as those of pristine calvarial bone, and its molecular cues suggested the occurrence of a remodeling process. Conclusion: Results of this study demonstrated that cell therapy with osteoblasts induced significant bone formation that exhibited the same quality as that of pre-existent bone. (AU)

• FAPESP's process: 14/26864-4 - Effect of Local Injections of Mesenchymal Stem Cells and Osteoblasts on Bone Regeneration
• Grantee: Gileade Pereira Freitas
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/21439-6 - Mesenchymal stem cells and osteoblasts: evaluation of molecular signatures and their potential for bone regeneration
• Grantee: Adalberto Luiz Rosa
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/54142-5 - Creation of a Multi-User Laboratory for the analysis of three-dimensional images of biological tissues and biomaterials (3D BIOLAB)
• Grantee: Adalberto Luiz Rosa
• Support Opportunities: Multi-user Equipment Program