Real-time redox monitoring of a nitrosyl ruthenium complex acting as NO-donor agent in a single A549 cancer cell with multiplex Fourier-transform infrared microscopy

Author(s)

Rodrigues, Fernando Postalli ^[1] ; Macedo, Lucyano J. A. ^[2] ; Maximo, Leandro N. C. ^{[1, 3]} ; Sales, Fernanda C. P. F. ^[2] ; da Silva, Roberto S. ^[1] ; Crespilho, Frank N. ^[2]

Affiliation

^[1] Univ Sao Paulo, Dept Phys & Chem, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Chem, BR-13560970 Sao Carlos, SP - Brazil

^[3] Inst Fed Educ Ciencia & Tecnol Goiano, Dept Chem, Campus Urutai, BR-75790000 Urutai, Go - Brazil

• Document type: Journal article
• Source: NITRIC OXIDE-BIOLOGY AND CHEMISTRY; v. 96, p. 29-34, MAR 1 2020.
• Web of Science Citations: 0

Abstract

Multiplex Fourier-transform infrared microscopy (FT-IR) helped to monitor trans-{[}Ru(NO) (NH3)(4) (isn)](3+) (I), uptake by A549 lung carcinoma cell, as well as the generation of its product, nitric oxide (NO), inside the cell. Chronoamperometry with NO-sensor and FT-IR showed that exogenous NADH and the A549 cell induced the NO release redox mechanism. Chemical imaging confirmed that (I) was taken up by the cell, and that its localization coincided with its consumption in the cellular environment within 15 min of exposure. The Ru-NO absorption band in the IR spectrum shifted from 1932 cm(-1), when NO was coordinated to Ru as [Ru-II-NO+](3+), to 1876 cm(-1), due the formation of reduced species [Ru-II-NO0](2+), a precursor of NO release. Futhermore, the mu FT-IR spectral profile demonstrated that, as a result of the NO action on the target, NO interacted with nucleic acids, which provided a biochemical response that is detectable in living cells. (AU)

• FAPESP's process: 13/07829-0 - Evaluation of Electrochemical Properties of Glucose/O2 Implantable Biofuel Cell
• Grantee: Fernanda Cristina Pena Ferreira Sales
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/12707-0 - Cytotoxicity and photo-cytotoxicity of new ruthenium-phthalocyanines compounds as nitric oxide and oxygen singlet producers in cancer cell lines. an innovative purpose for metal based drug
• Grantee: Roberto Santana da Silva
• Support type: Regular Research Grants
• FAPESP's process: 15/16672-3 - Development of High Performance Bioelectrodes for Application in Miniaturized Biofuel Cell
• Grantee: Frank Nelson Crespilho
• Support type: Regular Research Grants
• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 17/20493-2 - Study of metalloenzymes through electrochemistry coupled to vibrational spectroscopy
• Grantee: Lucyano Jefferson Alves de Macêdo
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/03964-3 - Ruthenium nitrosil complex as producer of nitric oxide and singlet oxygen: FOTOBIOLOGICAL studies, BICHEMICAL and their potential therapeutical aplication
• Grantee: Fernando Postalli Rodrigues
• Support type: Scholarships in Brazil - Post-Doctorate