Shifts in the bacterial community of saliva give insights on the relationship between obesity and oral microbiota in adolescents

Author(s)

de Andrade, Pedro Avelino Maia ^{[1, 2]} ; Giovani, Priscila Alves ^[3] ; Araujo, Darlle Santos ^[3] ; de Souza, Adijailton Jose ^[1] ; Pedroni-Pereira, Aline ^[3] ; Kantovitz, Kamila Rosamilia ^{[3, 4]} ; Andreote, Fernando Dini ^[1] ; Castelo, Paula Midori ^[5] ; Nociti-Jr, Francisco Humberto

Affiliation

^[1] Univ Sao Paulo Esalq USP, Dept Soil Sci, Coll Agr Luiz de Queiroz, Piracicaba, SP - Brazil

^[2] Univ Sao Paulo Esalq USP, Dept Genet, Coll Agr Luiz de Queiroz, Piracicaba, SP - Brazil

^[3] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Pediat Dent, Piracicaba, SP - Brazil

^[4] Sao Leopoldo Mandic Res Ctr, Dept Dent Mat, Campinas, SP - Brazil

^[5] Univ Fed Sao Paulo, Dept Biol Sci, Sao Paulo - Brazil

• Document type: Journal article
• Source: Archives of Microbiology; v. 202, n. 5 FEB 2020.
• Web of Science Citations: 0

Abstract

The current study aimed at the determination of the impact of obesity on the salivary microbiome in adolescents. Sixty subjects ranging 14-17 years old were enrolled (obese: n = 30-50% females, and normal weight: n = 30-50% females). Stimulated saliva was collected for denaturing gradient gel electrophoresis (DGGE) band patterns and massive 16S rRNA gene sequencing using the Ion Torrent platform. Overall, data analysis revealed that male subjects harbored a higher diverse salivary microbiome, defined by a significant higher richness (32.48 versus 26.74) and diversity (3.36 versus 3.20), higher Simpson values (0.96 versus 0.95) and distinct bacterial community structure considering either sex or condition (p < 0.05). Bacterial community fingerprinting analysis in human saliva showed a positive correlation with increased body mass index (BMI) in adolescents. Veillonella, Haemophilus and Prevotella occurrence was found to be affected by BMI, whereas Neisseria and Rothia occurrence was significantly impacted by sex in obese subjects. Our findings suggest that male and female adolescents may harbor a naturally distinct salivary microbiota and that obesity may specifically have an impact on their oral bacterial community. The potential dysbiotic oral microbiome in obese adolescents raises new insights on the etiology and prevention of future conditions in these populations. (AU)

• FAPESP's process: 16/02942-1 - Global Proteome Profile of Periodontal Ligament.
• Grantee: Priscila Alves Giovani
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/24804-4 - Evaluation of salivary composition and masticatory function in overweight and obese adolescents
• Grantee: Paula Midori Castelo Ferrua
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/04492-2 - Quality of life evaluation and the nutritional, salivary and masticatory characteristics of pre pubertal overweight and obese children
• Grantee: Paula Midori Castelo Ferrua
• Support Opportunities: Regular Research Grants