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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

First genome sequence of an isolate of hibiscus chlorotic ringspot virus from the Western hemisphere

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Author(s):
Ramos-Gonzalez, Pedro Luis [1] ; da Costa-Rodrigues, Mariane [1] ; Potsclam-Barro, Matheus [1] ; Chabi-Jesus, Camila [1, 2] ; Banguela-Castillo, Alexander [1] ; Harakava, Ricardo [1] ; Kitajima, Elliot W. [3] ; Freitas-Astua, Juliana [1, 4]
Total Authors: 8
Affiliation:
[1] Inst Biol, Lab Biol Mol Aplicada, BR-04014002 Vila Mariana, SP - Brazil
[2] PPG Microbiol Agr ESAL QUSP, BR-13418900 Piracicaba, SP - Brazil
[3] NAP MEPA ESALQ USP, BR-13418900 Piracicaba, SP - Brazil
[4] Embrapa Cassava & Fruits, R Embrapa, BR-44380000 Cruz Das Almas, BA - Brazil
Total Affiliations: 4
Document type: Journal article
Source: TROPICAL PLANT PATHOLOGY; v. 45, n. 2 FEB 2020.
Web of Science Citations: 0
Abstract

For the first time, the near-complete genome sequence of the betacarmovirus, hibiscus chlorotic ringspot virus (HCRSV) isolated from the Americas is disclosed. High throughput sequencing of the total RNA extract from a Hibiscus-rosa sinensis (L.) plant collected in Sao Paulo state, Brazil, revealed the genome sequence of HCRSV isolate SBO1, which is 3945 nucleotides long and shows 93.1% nucleotide sequence identity with HCRSV\_Singapore (X86448), considered the type member of the species. These two viruses display a similar genomic organization and potentially encode seven open reading frames (ORFs). In addition, a phylogenetic analysis based on a fragment with 557 nts of p38, the coat protein gene, from a cohort of 14 samples collected in Brazil revealed a no clearly defined segregation of South American isolates from those previously detected in geographical areas outside this continent. The practical consequences of the use of p38 ORF-derived amplicons for detection and variability studies of HCRSV are concisely discussed. (AU)

FAPESP's process: 14/08458-9 - Plant viruses transmitted by Brevipalpus mites (Acari: Tenuipalpidae) - BTV: survey, identification, molecular characterization, phylogeny; virus/vector/host relationhip; biology, taxonomy and management of the mite
Grantee:Elliot Watanabe Kitajima
Support type: Research Projects - Thematic Grants
FAPESP's process: 17/50222-0 - Understanding the molecular biology and ecology of plant-virus-vector relationships: towards sustainable, integrated virus management strategies
Grantee:Juliana de Freitas Astúa
Support type: Regular Research Grants
FAPESP's process: 17/50334-3 - Institutional research development plan (PDIp): modernization and adequacy of strategic multi-user units of the Instituto Biológico
Grantee:Ana Eugênia de Carvalho Campos
Support type: Research Grants - State Research Institutes Modernization Program
FAPESP's process: 19/09222-2 - Brevipalpus mite-borne virus infection in Hibiscus spp. in Brazil
Grantee:Mariane da Costa Rodrigues
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 16/01960-6 - Molecular characterization, phylogeny and evolución of Brevipalpus-transmitted viruses in Brazil
Grantee:Camila Chabi de Jesus
Support type: Scholarships in Brazil - Doctorate