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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nuclear export of replication protein A in the nonreplicative infective forms of Trypanosoma cruzi

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Pavani, Raphael S. [1, 2] ; de Lima, Loyze P. [1, 2] ; Lima, Andre A. [1, 2] ; Fernandes, Carlos A. H. [3, 4] ; Fragoso, Stenio P. [5] ; Calderano, Simone G. [6, 1] ; Elias, Maria Carolina [1, 2]
Total Authors: 7
[1] Inst Butantan, Ctr Toxins Immune Response & Cell Signaling CeTIC, Sao Paulo - Brazil
[2] Inst Butantan, Lab Ciclo Celular, Sao Paulo - Brazil
[3] Ecole Normale Super Paris Saclay, Lab Biol & Pharmacol Appl, Cachan - France
[4] Univ Estadual Paulista Julio de Mesquita Filho UN, Inst Biociencias, Dept Fis & Biofis, Botucatu, SP - Brazil
[5] Fiocruz MS, Inst Carlos Chagas, Curitiba, Parana - Brazil
[6] Inst Butantan, Lab Parasitol, Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: FEBS Letters; v. 594, n. 10 MAR 2020.
Web of Science Citations: 0

Replication protein A (RPA), a heterotrimeric complex, is the major single-stranded DNA binding protein in eukaryotes. Recently, we characterized RPA from Trypanosoma cruzi, showing that it is involved in DNA replication and DNA damage response in this organism. Better efficiency in differentiation from epimastigote to metacyclic trypomastigote forms was observed in TcRPA-2 subunit heterozygous knockout cells, suggesting that RPA is involved in this process. Here, we show that RPA cellular localization changes during the T. cruzi life cycle, with RPA being detected only in the cytoplasm of the metacyclic and bloodstream trypomastigotes. We also identify a nuclear export signal (NES) in the trypanosomatid RPA-2 subunit. Mutations in the negatively charged residues of RPA-2 NES impair the differentiation process, suggesting that RPA exportation affects parasite differentiation into infective forms. (AU)

FAPESP's process: 18/14432-3 - A network for an integrative biology in neglected diseases: bridging epigenetics, metabolism and cell cycle in pathogenic trypanosomatids
Grantee:Ariel Mariano Silber
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/02978-0 - Functional analysis of RPA complex in Trypanosoma cruzi and its involvement with telomeric DNA
Grantee:Raphael Souza Pavani
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 18/21785-0 - Analysis of Orc1/Cdc6-DNA interaction during the life cycle of Trypanosoma cruzi
Grantee:Loyze Paola Oliveira de Lima
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/50050-2 - How do common and diverged features of the replicative stress response shape the biology of TriTryp parasites?
Grantee:Maria Carolina Quartim Barbosa Elias Sabbaga
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
Grantee:Hugo Aguirre Armelin
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC