Pavani, Raphael S.
de Lima, Loyze P.
Lima, Andre A.
Fernandes, Carlos A. H.
Fragoso, Stenio P.
Calderano, Simone G.
Elias, Maria Carolina
Total Authors: 7
 Inst Butantan, Ctr Toxins Immune Response & Cell Signaling CeTIC, Sao Paulo - Brazil
 Inst Butantan, Lab Ciclo Celular, Sao Paulo - Brazil
 Ecole Normale Super Paris Saclay, Lab Biol & Pharmacol Appl, Cachan - France
 Univ Estadual Paulista Julio de Mesquita Filho UN, Inst Biociencias, Dept Fis & Biofis, Botucatu, SP - Brazil
 Fiocruz MS, Inst Carlos Chagas, Curitiba, Parana - Brazil
 Inst Butantan, Lab Parasitol, Sao Paulo - Brazil
Total Affiliations: 6
Web of Science Citations:
Replication protein A (RPA), a heterotrimeric complex, is the major single-stranded DNA binding protein in eukaryotes. Recently, we characterized RPA from Trypanosoma cruzi, showing that it is involved in DNA replication and DNA damage response in this organism. Better efficiency in differentiation from epimastigote to metacyclic trypomastigote forms was observed in TcRPA-2 subunit heterozygous knockout cells, suggesting that RPA is involved in this process. Here, we show that RPA cellular localization changes during the T. cruzi life cycle, with RPA being detected only in the cytoplasm of the metacyclic and bloodstream trypomastigotes. We also identify a nuclear export signal (NES) in the trypanosomatid RPA-2 subunit. Mutations in the negatively charged residues of RPA-2 NES impair the differentiation process, suggesting that RPA exportation affects parasite differentiation into infective forms. (AU)