Exercise training effects on SHR treated with dexamethasone: role of miRNAs
| Full text | |
| Author(s): |
Herrera, Naiara A.
[1]
;
Duchatsch, Francine
[1]
;
Tardelli, Lidieli P.
[1]
;
Dionisio, Thiago J.
[2]
;
Shinohara, Andre L.
[2]
;
Santos, Carlos F.
[2]
;
Amaral, Sandra Lia
[1, 3]
Total Authors: 7
|
| Affiliation: | [1] PIPGCF UFSCar UNESP, Joint Grad Program Physiol Sci, Rodovia Washington Luiz, Km 235 Monjolinho 676, BR-1356590 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Alameda Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil
[3] Sao Paulo State Univ, UNESP, Sch Sci, Dept Phys Educ, Av Eng Luiz Edmundo Carrijo Coube 14-01, BR-17033360 Bauru, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Molecular and Cellular Endocrinology; v. 505, APR 5 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Microcirculation maintenance is associated with microRNAs. Nevertheless, the role of microRNAs induced by training in preventing dexamethasone (DEX)-induced microvascular rarefaction remains unknown. The study aim was to investigate if training-induced microRNAs are able to improve microcirculation proteins and prevent DEX-induced microvascular rarefaction. Rats underwent training for 8 weeks and then were treated with DEX (50 mu g/kg per day, s.c.) for 14 days. Arterial pressure was measured and tibialis anterior (TA) muscle was collected for analyses. DEX induced hypertension concomitantly with capillary density loss (CD, -23.9%) and decrease of VEGF (-43.0%), p-AKT/AKT (-39.6%) and Bcl-2 (-23.0%) and an increase in caspase-3-cleaved protein level (+34.0%) in TA muscle. Training upregulated microRNA-126 expression (+13.1%), prevented VEGF (+61.4%), p-AKT/AKT (+37.7%), Bcl-2 (+7.7%) decrease and caspase-3-cleaved (-23.1%) increase associated with CD (+54.7%) reduction and hypertension prevention. MiRNA-126 upregulation, induced by training, plays a role in controlling microcirculation, which may be a potential target against DEX-induced microvascular rarefaction. (AU) | |
| FAPESP's process: | 18/06998-7 - Exercise training prevents rarefaction induced by dexamethasone: role of mitochondria |
| Grantee: | Naiara Araújo Herrera |
| Support type: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 17/14405-3 - Influence of aerobic physical training on cardiac remodeling of spontaneously hypertensive rats treated with dexamethasone |
| Grantee: | Francine Duchatsch Ribeiro de Souza |
| Support type: | Scholarships in Brazil - Master |
| FAPESP's process: | 16/12532-5 - Exercise training effects on SHR treated with dexamethasone: role of miRNAs |
| Grantee: | Naiara Araújo Herrera |
| Support type: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 17/00509-1 - Role of physical training on autonomic balance, arterial stiffness and rarefaction in spontaneously hypertensive rats treated with dexamethasone: role of miRNA |
| Grantee: | Sandra Lia do Amaral Cardoso |
| Support type: | Regular Research Grants |
| FAPESP's process: | 15/03965-2 - Role of the renin-angiotensin system in different oral inflammatory models: an experimental interdisciplinary and clinical approach |
| Grantee: | Carlos Ferreira dos Santos |
| Support type: | Research Projects - Thematic Grants |