MicroRNA-126 upregulation, induced by training, plays a role in controlling microcirculation in dexamethasone treated rats

Author(s)

Herrera, Naiara A. ^[1] ; Duchatsch, Francine ^[1] ; Tardelli, Lidieli P. ^[1] ; Dionisio, Thiago J. ^[2] ; Shinohara, Andre L. ^[2] ; Santos, Carlos F. ^[2] ; Amaral, Sandra Lia ^{[1, 3]}

Affiliation

^[1] PIPGCF UFSCar UNESP, Joint Grad Program Physiol Sci, Rodovia Washington Luiz, Km 235 Monjolinho 676, BR-1356590 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Alameda Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[3] Sao Paulo State Univ, UNESP, Sch Sci, Dept Phys Educ, Av Eng Luiz Edmundo Carrijo Coube 14-01, BR-17033360 Bauru, SP - Brazil

• Document type: Journal article
• Source: Molecular and Cellular Endocrinology; v. 505, APR 5 2020.
• Web of Science Citations: 0

Abstract

Microcirculation maintenance is associated with microRNAs. Nevertheless, the role of microRNAs induced by training in preventing dexamethasone (DEX)-induced microvascular rarefaction remains unknown. The study aim was to investigate if training-induced microRNAs are able to improve microcirculation proteins and prevent DEX-induced microvascular rarefaction. Rats underwent training for 8 weeks and then were treated with DEX (50 mu g/kg per day, s.c.) for 14 days. Arterial pressure was measured and tibialis anterior (TA) muscle was collected for analyses. DEX induced hypertension concomitantly with capillary density loss (CD, -23.9%) and decrease of VEGF (-43.0%), p-AKT/AKT (-39.6%) and Bcl-2 (-23.0%) and an increase in caspase-3-cleaved protein level (+34.0%) in TA muscle. Training upregulated microRNA-126 expression (+13.1%), prevented VEGF (+61.4%), p-AKT/AKT (+37.7%), Bcl-2 (+7.7%) decrease and caspase-3-cleaved (-23.1%) increase associated with CD (+54.7%) reduction and hypertension prevention. MiRNA-126 upregulation, induced by training, plays a role in controlling microcirculation, which may be a potential target against DEX-induced microvascular rarefaction. (AU)

• FAPESP's process: 18/06998-7 - EXERCISE TRAINING PREVENTS RAREFACTION INDUCED BY DEXAMETHASONE: ROLE OF MITOCHONDRIA
• Grantee: Naiara Araújo Herrera
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 17/14405-3 - Influence of aerobic physical training on cardiac remodeling of spontaneously hypertensive rats treated with dexamethasone
• Grantee: Francine Duchatsch Ribeiro de Souza
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 16/12532-5 - Exercise training effects on SHR treated with dexamethasone: role of miRNAs
• Grantee: Naiara Araújo Herrera
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/00509-1 - Role of physical training on autonomic balance, arterial stiffness and rarefaction in spontaneously hypertensive rats treated with dexamethasone: role of miRNA
• Grantee: Sandra Lia do Amaral Cardoso
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/03965-2 - Role of the renin-angiotensin system in different oral inflammatory models: an experimental interdisciplinary and clinical approach
• Grantee: Carlos Ferreira dos Santos
• Support Opportunities: Research Projects - Thematic Grants