Genosensor made with a self-assembled monolayer matrix to detect MGMT gene methylation in head and neck cancer cell lines

Author(s):Show less -

Carr, Olivia ^[1] ; Raymundo-Pereira, Paulo A. ^[1] ; Shimizu, Flavio M. ^{[1, 2]} ; Sorroche, Bruna Pereira ^[3] ; Melendez, Matias Eliseo ^[3] ; Pedro, Rafael de Oliveira ^[1] ; Miranda, Paulo B. ^[1] ; Carvalho, Andre Lopes ^[3] ; Reis, Rui M. ^{[4, 3, 5]} ; Arantes, Lidia M. R. B. ^[3] ; Oliveira, Jr., Osvaldo N. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sao Carlos Inst Phys, CP 369, BR-13566590 Sao Carlos - Brazil

^[2] Brazilian Ctr Res Energy & Mat CNPEM, Nanotechnol Natl Lab LNNano, BR-13083970 Campinas, SP - Brazil

^[3] Barretos Canc Hosp, Mol Oncol Res Ctr, BR-14784400 Barretos - Brazil

^[4] ICVS 3Bs PT Govt Associate Lab, Braga - Portugal

^[5] Univ Minho, Life & Hlth Sci Res Inst ICVS, Sch Med, Braga - Portugal

• Document type: Journal article
• Source: Talanta; v. 210, APR 1 2020.
• Web of Science Citations: 0

Abstract

DNA methylation is involved in the oncogenesis of head and neck squamous cell carcinoma and could be used for early detection of cancer to increase the chances of cure, but unfortunately diagnosis is usually made at late stages of the disease. In this work we developed genosensors to detect DNA methylation of the MGMT gene in head and neck cancer cell lines. The probe for MGMT promoter methylation was immobilized on gold electrodes modified with 11-mercaptoundecanoic acid (11-MUA) self-assembled monolayers (SAM). Detection was performed with electrochemical impedance spectroscopy, with clear distinction between methylated and non-methylated DNA from head and neck cell lines. The genosensor is sensitive with a low detection limit of 0.24 x 10(-12) mol L-1. In addition, the cell lines FaDu, JHU28 and SCC25 for the MGMT gene, could be distinguished from the HN13 cell line which has a high degree of MGMT methylation (97%), thus confirming the selectivity. Samples with different percentages of MGMT DNA methylation could be separated in multidimensional projections using the visualization technique interactive document mapping (IDMAP). The genosensor matrix and the immobilization procedures are generic, and can be extended to other DNA methylation biomarkers. (AU)

• FAPESP's process: 17/03503-4 - Molecular interaction between chitosan and cell membrane models investigated by non-linear vibrational spectroscopy
• Grantee: Rafael de Oliveira Pedro
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 12/15543-7 - Biosensors to detect Escherichia coli using the concept of an extended electronic tongue
• Grantee: Flavio Makoto Shimizu
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/02819-0 - Interaction of chitosans and surfactants with cell membrane models investigated by nonlinear vibrational spectroscopy
• Grantee: Paulo Barbeitas Miranda
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/12990-0 - Methylation as a response modulation mechanism to EGFR inhibitors in Head and Neck Carcinoma
• Grantee: Lidia Maria Rebolho Batista Arantes
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/13390-8 - PLATFORM: liquid biopsy for melanomas
• Grantee: Bruna Pereira Sorroche
• Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training
• FAPESP's process: 16/01919-6 - Design and fabrication of nanostructured flexible devices for biomarkers detection
• Grantee: Paulo Augusto Raymundo Pereira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral