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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genosensor made with a self-assembled monolayer matrix to detect MGMT gene methylation in head and neck cancer cell lines

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Carr, Olivia [1] ; Raymundo-Pereira, Paulo A. [1] ; Shimizu, Flavio M. [1, 2] ; Sorroche, Bruna Pereira [3] ; Melendez, Matias Eliseo [3] ; Pedro, Rafael de Oliveira [1] ; Miranda, Paulo B. [1] ; Carvalho, Andre Lopes [3] ; Reis, Rui M. [4, 3, 5] ; Arantes, Lidia M. R. B. [3] ; Oliveira, Jr., Osvaldo N. [1]
Total Authors: 11
[1] Univ Sao Paulo, Sao Carlos Inst Phys, CP 369, BR-13566590 Sao Carlos - Brazil
[2] Brazilian Ctr Res Energy & Mat CNPEM, Nanotechnol Natl Lab LNNano, BR-13083970 Campinas, SP - Brazil
[3] Barretos Canc Hosp, Mol Oncol Res Ctr, BR-14784400 Barretos - Brazil
[4] ICVS 3Bs PT Govt Associate Lab, Braga - Portugal
[5] Univ Minho, Life & Hlth Sci Res Inst ICVS, Sch Med, Braga - Portugal
Total Affiliations: 5
Document type: Journal article
Source: Talanta; v. 210, APR 1 2020.
Web of Science Citations: 0

DNA methylation is involved in the oncogenesis of head and neck squamous cell carcinoma and could be used for early detection of cancer to increase the chances of cure, but unfortunately diagnosis is usually made at late stages of the disease. In this work we developed genosensors to detect DNA methylation of the MGMT gene in head and neck cancer cell lines. The probe for MGMT promoter methylation was immobilized on gold electrodes modified with 11-mercaptoundecanoic acid (11-MUA) self-assembled monolayers (SAM). Detection was performed with electrochemical impedance spectroscopy, with clear distinction between methylated and non-methylated DNA from head and neck cell lines. The genosensor is sensitive with a low detection limit of 0.24 x 10(-12) mol L-1. In addition, the cell lines FaDu, JHU28 and SCC25 for the MGMT gene, could be distinguished from the HN13 cell line which has a high degree of MGMT methylation (97%), thus confirming the selectivity. Samples with different percentages of MGMT DNA methylation could be separated in multidimensional projections using the visualization technique interactive document mapping (IDMAP). The genosensor matrix and the immobilization procedures are generic, and can be extended to other DNA methylation biomarkers. (AU)

FAPESP's process: 17/03503-4 - Molecular interaction between chitosan and cell membrane models investigated by non-linear vibrational spectroscopy
Grantee:Rafael de Oliveira Pedro
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 12/15543-7 - Biosensors to detect Escherichia coli using the concept of an extended electronic tongue
Grantee:Flavio Makoto Shimizu
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 18/02819-0 - Interaction of chitosans and surfactants with cell membrane models investigated by nonlinear vibrational spectroscopy
Grantee:Paulo Barbeitas Miranda
Support Opportunities: Regular Research Grants
FAPESP's process: 15/12990-0 - Methylation as a response modulation mechanism to EGFR inhibitors in Head and Neck Carcinoma
Grantee:Lidia Maria Rebolho Batista Arantes
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
Grantee:Osvaldo Novais de Oliveira Junior
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 19/13390-8 - PLATFORM: liquid biopsy for melanomas
Grantee:Bruna Pereira Sorroche
Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training
FAPESP's process: 16/01919-6 - Design and fabrication of nanostructured flexible devices for biomarkers detection
Grantee:Paulo Augusto Raymundo Pereira
Support Opportunities: Scholarships in Brazil - Post-Doctoral