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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Prospecting for l-arabinose/d-xylose symporters from Pichia guilliermondii and Aureobasidium leucospermi

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Author(s):
da Silva, Ronivaldo Rodrigues [1] ; Prista, Catarina [2, 3] ; Loureiro Dias, Maria Conceicao [2] ; Boscolo, Mauricio [1] ; da Silva, Roberto [1] ; Gomes, Eleni [1]
Total Authors: 6
Affiliation:
[1] IBILCE UNESP, Inst Biociencias Letras & Ciencias Exatas, R Cristovao 51-50 Colombo, 2265 Jd Nazareth, Sao Jose Do Rio Preto, SP - Brazil
[2] Univ Lisbon, Inst Super Agron, LEAF Linking Landscape Environm Agr & Food, P-1349017 Lisbon - Portugal
[3] Univ Lisbon, Inst Super Agron, DRAT, P-1349017 Lisbon - Portugal
Total Affiliations: 3
Document type: Journal article
Source: Brazilian Journal of Microbiology; v. 51, n. 1, p. 145-150, MAR 2020.
Web of Science Citations: 0
Abstract

With the strong trend toward sustainable technologies, such as the gradual substitution of fossil fuel consumption, improvement in the utilization of sugars from lignocellulosic biomass appears to be an alternative for bioenergy. However, from a number of C-5 sugars, few are used in fermentative processes for ethanol production. One of the reasons is because wild-type Saccharomyces cerevisiae is unable to efficiently co-utilize hexoses and pentoses via specific transporters for each type of sugar. Thus, a system of pentose uptake that is not modulated by d-glucose is required. Here, we were able to identify the presence of sugar/H+ symporters for d-xylose and l-arabinose, especially for Pichia guilliermondii, where an uptake of d-glucose via symporter was not detected. The best d-xylose uptake route in P. guilliermondii exhibited a K-M of 48 mM and V-MAX of 0.48 mmol h(-1) g(-1) at the early stationary phase (24 h). For l-arabinose, the best route of uptake exhibited a K-M of 109 mM and V-MAX of 0.8 mmol h(-1) g(-1) on log phase (12 h). The highest kinetic uptake was observed when the final pH of the medium was below 7. In general, an alkaline medium limited the expression of symporters. The results obtained in this study will help in the further investigation of these symporters through their overexpression in engineered S. cerevisiae. (AU)

FAPESP's process: 18/09238-3 - Investigating the membrane transport and assimilation of L-arabinose and xylose by the yeasts Aureobasidium leucospermi LB86, Candida oleophila G10.1, Issachenkia terricola G20 and Pichia guilliermondii G1.2
Grantee:Ronivaldo Rodrigues da Silva
Support type: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 17/06399-3 - Investigating the membrane transport and assimilation of L-arabinose and xylose by the yeasts leucospermi LB86, Candida oleophila G10.1, Issachenkia terricola G20 and Pichia guilliermondii G1.2
Grantee:Ronivaldo Rodrigues da Silva
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/06066-4 - Native xylose and arabinose yeasts-assimilating: study of the assimilation and metabolization of the pentoses and use of these sugars from lignocellulosic biomass for obtainment bio-products
Grantee:Eleni Gomes
Support type: Regular Research Grants