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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Rickettsia rickettsii (Rickettsiales: Rickettsiaceae) Infecting Amblyomma sculptum (Acari: Ixodidae) Ticks and Capybaras in a Brazilian Spotted Fever-Endemic Area of Brazil

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Author(s):
Costa, Francisco B. [1, 2] ; Gerardi, Monize [1] ; Binder, Lina de C. [1] ; Benatti, Hector R. [1] ; de Azevedo Serpa, Maria Carolina [1] ; Lopes, Beatriz [3] ; Luz, Hermes R. [1, 4] ; Ferraz, Katia M. P. M. B. [3] ; Labruna, Marcelo B. [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, BR-05508270 Sao Paulo, SP - Brazil
[2] Univ Estadual Maranhao, Dept Patol, Programa Posgrad Ciencia Anim, BR-65055310 Sao Luis, Maranhao - Brazil
[3] Univ Sao Paulo, Dept Ciencias Florestais, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP - Brazil
[4] Univ Fed Maranhao, Dept Patol, Programa Posgrad Biotecnol RENORBIO, BR-65500000 Sao Luis, Maranhao - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Medical Entomology; v. 57, n. 1, p. 308-311, JAN 2020.
Web of Science Citations: 2
Abstract

The bacterium Rickettsia rickettsii is the agent of Brazilian spotted fever (BSF), a highly fatal disease that is transmitted in Brazil mainly by the tick Amblyomma sculptum Berlese, which uses capybaras (Hydrochoerus hydrochaeris Linnaeus) as major hosts. In 2015, we captured nine capybaras in a BSF-endemic area of southeastern Brazil. From each capybara, we collected blood sera that were tested through the immunofluorescence assay using Rickettsia spp. antigens, and A. sculptum ticks, processed for isolation of R. rickettsii through guinea pig inoculation. All capybaras (100%) were seroreactive to Rickettsia spp., with highest titers to R. rickettsii. A total of 166 A. sculptum ticks were macerated and inoculated into nine guinea pigs, from which only one presented high fever and seroconverted to R. rickettsii. Blood from this febrile animal was inoculated into additional guinea pigs (guinea pig passages), which also became febrile and seroconverted, or when euthanized during the fever period, their internal organs (spleen, lung) were shown to contain R. rickettsii DNA. The present rickettsial isolate has been maintained cryopreserved as infected guinea pig organs. There was at least one R. rickettsii-infected tick among the 166 macerated ticks, giving a minimal infection rate of 0.6% (1/166). This infection rate is within the range of previous studies, which reported that only 0.05% to at most 1.28% A. sculptum ticks were infected by R. rickettsii in other BSF-endemic areas. These low infection rates support the low incidence of BSF, despite of A. sculptum being the most frequent human-biting tick in southeastern Brazil. (AU)

FAPESP's process: 14/23176-0 - Population structure and dynamics of free-living capybara associated with tick infestation and Rickettsia spp. infection
Grantee:Francisco Borges Costa
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/18046-7 - Capybaras, ticks, and spotted fever
Grantee:Marcelo Bahia Labruna
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/27078-2 - Diagnosis and monitoring of free-living capybaras - 1
Grantee:Beatriz Lopes
Support Opportunities: Scholarships in Brazil - Scientific Initiation