Synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase immobilized on a magnetic polymer support in continuous flow

Author(s)

da Silva, Mateus V. C. ^[1] ; Souza, Amanda B. ^[1] ; de Castro, Heizir F. ^[1] ; Aguiar, Leandro G. ^[1] ; de Oliveira, Pedro C. ^[1] ; de Freitas, Larissa ^[1]

Affiliation

^[1] Univ Sao Paulo, Engn Sch Lorena, Dept Chem Engn, BR-12602810 Lorena, SP - Brazil

• Document type: Journal article
• Source: Bioprocess and Biosystems Engineering; v. 43, n. 4, p. 615-623, APR 2020.
• Web of Science Citations: 0

Abstract

This study investigated the synthesis of 2-ethylhexyl oleate catalyzed by Candida antarctica lipase immobilized on magnetic poly(styrene-co-divinylbenzene) particles in a continuous packed-bed bioreactor. Runs were carried out in a solvent-free system at 50 degrees C. The performance of the reactor was evaluated for substrates composed by oleic acid and 2-ethylhexanol at five molar ratios (1:4-4:1), determining its operation limits in terms of substrate flow rate. The system performance was quantified for three different flow rates corresponding to space-time between 3 and 12 h. For each condition, the influence of the space-time in the ester formation, esterification yield and productivity was determined. The molar ratio of acid-to-alcohol interfered, in a remarkable way, in the formation of 2-ethylhexyl oleate and the best performance was attained for substrate at equimolar ratio running at 12 h space-time. Under this condition, average 2-ethylhexyl oleate concentration was 471.65 +/- 2.98 g L-1 which corresponded to ester productivity of 23.16 +/- 0.49 mmol g(-1) L-1 h(-1). This strategy also gave high biocatalyst operational stability, revealing a half-life time of 2063 h. A model based on the ping-pong Bi-Bi mechanism was developed to describe the kinetics of the esterification reaction and validated using experimental data. The goodness of fit of the model was satisfactory (R-2 = 0.9310-0.9952). (AU)

• FAPESP's process: 17/04934-9 - Synthesis and characterization of magnetized poly (styrene-co-divinylbenzene) particles to be used as support for enzyme immobilization
• Grantee: Cintia Maria Rodrigues Rosa
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 16/17833-3 - Emollient esters synthesis catalyzed by immobilized lipase on styrene-based polymers with magnetic properties
• Grantee: Larissa de Freitas
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
• Grantee: Roberto de Campos Giordano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants