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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cytotoxicity and release ions of endodontic sealers incorporated with a silver and vanadium base nanomaterial

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Teixeira, Ana Beatriz Vilela [1] ; de Castro, Denise Tornavoi [1] ; Schiavon, Marco Antonio [2] ; dos Reis, Andrea Candido [1]
Total Authors: 4
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Dent Mat & Prosthesis, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil
[2] Univ Fed Sao Joao del Rei, Dept Nat Sci, Praca Dom Helvecio 74, Campus Dom Bosco, BR-36301160 Sao Joao Del Rei, MG - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ODONTOLOGY; MAR 2020.
Web of Science Citations: 0

The modification of endodontic sealers with nanoparticles to confer antimicrobial activity allow greater effect, with interaction at a molecular level. The nanostructured silver vanadate decorated with silver nanoparticles (AgVO3) is a nanomaterial unprecedented in dentistry for this application. This study incorporated the AgVO3 into three endodontic sealers of different compositions and evaluate the cytotoxicity and release of compounds. The groups of commercially available AH Plus, Sealer 26, and Endomethasone N and groups of the same sealers with incorporated AgVO3 (at concentrations 2.5, 5, 10%) were prepared, and extracts of the specimens were obtained for 24 h. The cell viability (cytotoxicity) of human gingival fibroblasts (HGF) was assessed after 24 h, 7 and 14 days. Silver (Ag+) and vanadium (V4+/V5+) ion release was quantified after 24 h by ICP-MS. Data were analyzed by Kruskal-Wallis and Dunn's post-hoc (alpha = 0.05). The cell viability was inversely proportional to treatment time. The Sealer 26 and Endomethasone N groups were cytotoxic for HGF cells, regardless of the incorporation of the AgVO3 (p > 0.05), and the incorporation reduced cell viability of AH Plus (p < 0.05). The release of ions was proportional to the concentration of AgVO3. AH Plus released more Ag+ ions, and Sealer 26 and Endomethasone N releases more V4+/V5+ ions. In conclusion, it was not possible to confirm the influence of AgVO3 on HGF cell viability to Sealer 26 and Endomethasone N, however, nanomaterial influenced cell-viability to AH Plus, so the commercial sealers can be cytotoxic in synergy with the nanomaterial. The release of Ag+ and V4+/V5+ was proportional to the AgVO3 incorporated. (AU)

FAPESP's process: 17/04667-0 - Evaluation of endodontic sealers incorporated with silver vanadate nanostructured decorated with silver nanoparticles for toxicity, antimicrobial and physicochemical properties
Grantee:Ana Beatriz Vilela Teixeira
Support type: Scholarships in Brazil - Doctorate