Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Frizzled 6 disruption suppresses osteoblast differentiation induced by nanotopography through the canonical Wnt signaling pathway

Full text
Author(s):
Flores Abuna, Rodrigo Paolo [1] ; Oliveira, Fabiola Singaretti [1] ; Adolpho, Leticia Faustino [1] ; Fernandes, Roger Rodrigo [1] ; Rosa, Adalberto Luiz [1] ; Beloti, Marcio Mateus [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Bone Res Lab, Av Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Cellular Physiology; APR 2020.
Web of Science Citations: 0
Abstract

This study aimed to investigate if wingless-related integration site (Wnt) signaling participates in the high osteogenic potential of titanium with nanotopography (Ti-Nano). We showed that among the several components of the Wnt signaling pathway, Frizzled 6 (Fzd6) was the transcript most intensely modulated by nanotopography compared with the untreated Ti surface (Ti-Machined). Then, we investigated whether and how Fzd6 participates in the regulation of osteoblast differentiation caused by nanotopography. The Fzd6 silencing with CRISPR-Cas9 transfection in MC3T3-E1 cells induced a more pronounced inhibition of osteoblast differentiation of cells cultured on nanotopography than those cultured on Ti-Machined. The analysis of the expression of calcium-calmodulin-dependent protein kinase II and beta-catenin demonstrated that Fzd6 disruption inhibited the osteoblast differentiation induced by Ti-Nano by preventing the activation of Wnt/beta-catenin but not that of Wnt/Ca2+ signaling, which is usually triggered by the receptor Fzd6. These findings elucidate the biological function of Fzd6 as a receptor that triggers Wnt/beta-catenin signaling and the cellular mechanisms modulated by nanotopography during osteoblast differentiation. (AU)

FAPESP's process: 16/14171-0 - Role of Wnt Signaling Pathways in the Osteogenic Potential of Titanium Surface with Nanotopography
Grantee:Márcio Mateus Beloti
Support type: Regular Research Grants
FAPESP's process: 16/14711-4 - Role of Wnt Signaling Pathways in the Osteogenic Potential of Titanium Surface with Nanotopography
Grantee:Rodrigo Paolo Flores Abuna
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 18/17356-6 - Effect of titanium surface with nanotopography on the interaction between osteoblasts and osteoclasts
Grantee:Márcio Mateus Beloti
Support type: Regular Research Grants