Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products

Full text
Author(s):
Sigrist, Renata [1] ; Paulo, Bruno S. [1] ; Angolini, Celio F. F. [2] ; De Oliveira, Luciana G. [1]
Total Authors: 4
Affiliation:
[1] Univ Estadual Campinas, Inst Chem, Dept Organ Chem, UNICAMP, Campinas, SP - Brazil
[2] Fed Univ ABC UFABC, Ctr Nat & Human Sci, Santo Andre, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS; n. 157 MAR 2020.
Web of Science Citations: 0
Abstract

The chemical space covered by natural products is immense and widely unrecognized. Therefore, convenient methodologies to perform wide-ranging evaluation of their functions in nature and potential human benefits (e.g., for drug discovery applications) are desired. This protocol describes the combination of genome mining (GM) and molecular networking (MN), two contemporary approaches that match gene cluster-encoded annotations in whole genome sequencing with chemical structure signatures from crude metabolic extracts. This is the first step towards the discovery of new natural entities. These concepts, when applied together, are defined here as MS-guided genome mining. In this method, the main components are previously designated (using MN), and structurally related new candidates are associated with genome sequence annotations (using GM). Combining GM and MN is a profitable strategy to target new molecule backbones or harvest metabolic profiles in order to identify analogues from already known compounds. (AU)

FAPESP's process: 19/10564-5 - Assessing the beneficial aspects of endophytic actinobacteria: expanding the Brazilian microorganisms genome codified natural products
Grantee:Luciana Gonzaga de Oliveira
Support type: Regular Research Grants
FAPESP's process: 14/50249-8 - Green chemistry: sustainable synthetic methods employing benign solvents, safer reagents, and bio-renewable feedstock
Grantee:Arlene Gonçalves Corrêa
Support type: Research Grants - Research Centers in Engineering Program
FAPESP's process: 19/08853-9 - Chemical Ecology of plant-microorganisms interactions studies for a sustainable agriculture
Grantee:Célio Fernando Figueiredo Angolini
Support type: Regular Research Grants
FAPESP's process: 15/01013-4 - Mining a t1PKS-transAT PKS-NRPS in Streptomyces sp. genome
Grantee:Renata Sigrist
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 13/12598-8 - Mining a of t1PKS-transAT PKS-NRPS in Streptomyces sp.
Grantee:Renata Sigrist
Support type: Scholarships in Brazil - Master
FAPESP's process: 14/12727-5 - Genome mining in Streptomyces
Grantee:Luciana Gonzaga de Oliveira
Support type: Regular Research Grants