Carnitine Diminishes Etoposide Toxic Action on Spe... - BV FAPESP
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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Carnitine Diminishes Etoposide Toxic Action on Spermatogonial Self-renewal and Sperm Production in Adult Rats Treated in the Prepubertal Phase

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Author(s):
Okada, Fatima Kazue [1] ; Stumpp, Taiza [1] ; Miraglia, Sandra Maria [1]
Total Authors: 3
Affiliation:
[1] Univ Fed Sao Paulo, Dev Biol Lab, 740 Botucatu, Ed Leitao da Cunha, 2nd Floor, BR-04023900 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY; v. 68, n. 5 MAR 2020.
Web of Science Citations: 0
Abstract

The aim of this study was to investigate carnitine action against negative effects of etoposide on stem/progenitor spermatogonia and on sperm production. Carnitine (250 mg/kg body weight/day) and etoposide (5 mg/kg body weight/day) were administered from 25-days postpartum to 32-days postpartum. Testes were collected at 32-days postpartum, 64-days postpartum, and 127-days postpartum, and submitted to the immuno-labeling of UTF1, SOX2, and PLZF proteins to identify undifferentiated spermatogonia populations. At 127-days postpartum, sperm were collected for analysis. Carnitine+etoposide group showed a higher numerical density of spermatogonia labeled for all studied proteins at 64-days postpartum (critical age) compared to the etoposide group. Moreover, there was an improvement of spermatic parameters and sperm DNA integrity in rats of the carnitine+etoposide group in comparison with rats of the etoposide group. The results suggest that carnitine improves the self-renewal of undifferentiated spermatogonia and promotes a partial protection on them, alleviating the etoposide harmful late effects and leading to an enhancement of the sperm parameters in adulthood: (AU)

FAPESP's process: 11/12371-8 - Immunohistochemical and stereological study on spermatogonial cell populations of rats treated with etoposide and carnitine in the prepuberty
Grantee:Sandra Maria Miraglia Valdeolivas
Support Opportunities: Regular Research Grants