Phylogenomic assessment of drug-resistant Mycobacterium tuberculosis strains from Beira, Mozambique

Author(s):Show less -

Namburete, Evangelina Inacio ^{[1, 2]} ; Dippenaar, Anzaan ^[3] ; Conceicao, Emilyn Costa ^[4] ; Feliciano, Cinara ^[1] ; do Nascimento, Margarida Maria Passeri ^[1] ; Peronni, Kamila Chagas ^[5] ; Silva Jr, Wilson Araujo ; Ferro, Josefo Joao ^[2] ; Harrison, Lee H. ^[6] ; Warren, Robin Mark ^[3] ; Bollela, Valdes Roberto ^[1]

Affiliation

^[1] Univ Sao Paulo FMRP USP, Mycobacteria Res Lab, Clin Hosp, Ribeirao Preto Med Sch, Ribeirao Preto, SP - Brazil

^[2] Catholic Univ Mozamb, Fac Hlth Sci, Beira - Mozambique

^[3] Stellenbosch Univ, Fac Med & Hlth Sci, DST NRF Ctr Excellence Biomed TB Res, Div Mol Biol & Human Genet, SAMRC Ctr TB Res, Stellenbosch - South Africa

^[4] Univ Fed Rio de Janeiro, Inst Microbiol Paulo de Goes, Av Carlos Chagas Filho, 373 Bloco I Cidade Univ, BR-21941970 Rio De Janeiro, RJ - Brazil

^[5] Univ Sao Paulo FMRP USP, Clin Hosp Ribeirao Preto Med Sch, Ctr Med Genom, Ribeirao Preto, SP - Brazil

^[6] Univ Pittsburgh, Infect Dis Epidemiol Res Unit, Pittsburgh, PA - USA

• Document type: Journal article
• Source: TUBERCULOSIS; v. 121, MAR 2020.
• Web of Science Citations: 0

Abstract

Background: Mozambique is a high-burden tuberculosis (TB) country where TB/HIV co-infection and drug resistant TB (DR-TB) incidence is increasing. Whole genome sequencing (WGS) comprehensively describes the molecular epidemiology of TB, allows prediction of DR-TB phenotypes, lineages strains identification and better understanding of transmission chains. Objective: To describe genetic diversity of DR-TB Mycobacterium tuberculosis isolated in Beira, Mozambique. Methods: Descriptive cross-sectional study with 35 M. tuberculosis isolates, resistant to at least one first-line drug on molecular drug-susceptibility tests (DST). Variant identification, DR prediction and phylogenetic analysis provided by WGS, drug-susceptibility pattern compared to line-probe assay (LPA): Genotype MTBDR (TM) plus and MTBDR (TM) sl. Findings: Lineage 4 (L4) was the most prevalent: 25 (71.4%) isolates; 5 (14.3%) L1 and 5 (14.3%) L2. WGS showed 33/35 (94.3%) isolates resistant to at least one drug, two pan-susceptible isolates that were previously diagnosed as DR-TB with genotype MTBDRplus. Concordance between WGS and LPA: 88.6% for isoniazid (INH), 85.7% to rifampicin (RPM), 91.4% for quinolones and 100% to second line injectable drugs. There were three possible TB transmission chains, 10 strains showing recent transmission. Conclusion: WGS provided reliable information about the most frequent lineages related to DR-TB in Beira, Mozambique: L4.3 (LAM), L2 (Beijing) and L1 (EAI) and possible recent transmission chain. (AU)

• FAPESP's process: 15/13333-3 - Molecular epidemiology of multidrug resistant tuberculosis through whole genome sequencing
• Grantee: Valdes Roberto Bollela
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/07341-1 - Evaluation of tuberculosis dynamics transmission among incarcerated people using whole genome sequencing of Mycobacterium tuberculosis.
• Grantee: Valdes Roberto Bollela
• Support Opportunities: Regular Research Grants