Proteolytic enzymes positively modulated the physicochemical and antioxidant properties of spent yeast protein hydrolysates

Author(s)

Marson, Gabriela Vollet ^{[1, 2]} ; Soares de Castro, Ruann Janser ^[3] ; da Costa Machado, Mariana Teixeira ^[4] ; Zandonadi, Flavia da Silva ^[5] ; de Freitas Queiroz Barros, Helena Dias ^[6] ; Marostica Junior, Mario Roberto ^[6] ; Sussulini, Alessandra ^[5] ; Hubinger, Miriam Dupas ^[1]

Affiliation

^[1] Univ Estadual Campinas, Dept Food Engn, Sch Food Engn, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

^[2] Univ Montpellier, Inst Europeen Membranes, CNRS, ENSCM, CC 047, 2 Pl Eugene Bataillon, F-34095 Montpellier - France

^[3] Univ Estadual Campinas, Dept Food Sci, Sch Food Engn, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

^[4] Univ Fed Rural Rio de Janeiro, Dept Food Technol, Rodovia BR 465, Km 7, BR-23890000 Seropedica, RJ - Brazil

^[5] Univ Estadual Campinas, Inst Chem, Dept Analyt Chem, BR-13083970 Campinas, SP - Brazil

^[6] Univ Estadual Campinas, Dept Food & Nutr, Sch Food Engn, Rua Monteiro Lobato 80, BR-13083862 Campinas, SP - Brazil

• Document type: Journal article
• Source: Process Biochemistry; v. 91, p. 34-45, APR 2020.
• Web of Science Citations: 7

Abstract

The spent brewer yeast (SBY) is a by-product with great potential for the production of peptides. Protein hydrolysates of SBY with improved physicochemical and antioxidant properties were produced by a system containing mixtures of proteases (Alcalase (TM), Brauzyn (R) and Protamex (TM)). The amount of solids and crude proteins recovered from the raw material after the protein hydrolysis was, in average, 57% and 70% (w/w), respectively. Hydrolysis resulted in intermediate degrees of hydrolysis (15%), promoting the release of hydrophobic residues (8% higher related to the control) with antioxidant properties. The presence of all three enzymes influenced the degree of hydrolysis but the darkening and browning of the hydrolysates were mainly affected by Protamex (TM). Most of the hydrolyzed proteins presented a molecular weight (MW) of 35 kg mol(-1), as confirmed after membrane separation and electrophoresis. The membrane fractionation step using 30 kg moL(-1) membranes effectively produced peptide-enriched fractions of 30-35 kg moL(-1). In addition, it was possible to correlate the degree of hydrolysis, solids release, color parameters L{*}, Browning Index and antioxidant properties by FRAP and DPPH in function of the hydrolysis conditions, showing the potential use of SBY protein hydrolysates as a source of peptides in value-added functional foods. (AU)

• FAPESP's process: 06/03263-9 - Process, packaging, edible coatings application and final quality evaluation of some high moisture tropical fruits
• Grantee: Miriam Dupas Hubinger
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/51707-1 - Understanding the emulsion behaviour of fermentation mixtures in the microbial production of diesel and jet-like biofuels
• Grantee: Rosiane Lopes da Cunha
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• FAPESP's process: 09/54137-1 - Acquisition of a particle size/distribution analyzer, a zeta potential measurement system, a spray dryer, and an ultra-high-pressure liquid chromatograph/mass spectrometer
• Grantee: Miriam Dupas Hubinger
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 16/18465-8 - Membrane concentration and fracionation of brewer spent yeast protein hydrolyzate
• Grantee: Gabriela Vollet Marson
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 15/50333-1 - COBRA: a novel compound discovery pipeline for rapid and cost-effective identification of bioactives with health beneficial effects in Brazilian crops
• Grantee: Glaucia Maria Pastore
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/04067-6 - Membrane concentration and fractionation of brewer spent yeast protein hydrolysate
• Grantee: Gabriela Vollet Marson
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• FAPESP's process: 17/04231-8 - Supercritical fractionation of the phenolics present in the Jabuticaba peel (Plinia cauliflora) and evaluation of the digestibility and bioavailability
• Grantee: Helena Dias de Freitas Queiroz Barros
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/24351-2 - Applications of image analyses and NIR spectroscopy for quality assessment and authentication of food products
• Grantee: Douglas Fernandes Barbin
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 11/06083-0 - Emulsification by microchannels
• Grantee: Rosiane Lopes da Cunha
• Support Opportunities: Regular Research Grants