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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Interference of goethite in the effects of glyphosate and Roundup (R) on ZFL cell line

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da Silva, Natara D. G. [1] ; Carneiro, Cristiane E. A. [2] ; Campos, Estefania V. R. [3] ; de Oliveira, Jhones L. [3] ; Risso, Wagner E. [4] ; Fraceto, Leonardo F. [3] ; Zaia, Dimas A. M. [2] ; Martinez, Claudia B. R. [1, 4]
Total Authors: 8
[1] Univ Estadual Londrina, Programa Posgrad Ciencias Biol, Londrina, Parana - Brazil
[2] Univ Estadual Londrina, Dept Quim, Londrina, Parana - Brazil
[3] Univ Estadual Paulista, Dept Engn Ambiental, UNESP, Sorocaba, SP - Brazil
[4] Univ Estadual Londrina, Dept Ciencias Fisiol, BR-86057970 Londrina, Parana - Brazil
Total Affiliations: 4
Document type: Journal article
Source: TOXICOLOGY IN VITRO; v. 65, JUN 2020.
Web of Science Citations: 0

Goethite (alpha-FeOOH) brings important perspectives in environmental remediation, as, due to its physicochemical properties, this iron oxide can adsorb a wide variety of compounds, including glyphosate. This study aimed to evaluate the effects of goethite nanoparticles (NPs), glyphosate (Gly), Roundup (R) (Rd), and co-exposures (Gly + NPs and Rd + NPs) on zebrafish liver cell line (ZFL). ZFL cells were exposed to NPs (1, 10, and 100 mg L-1), Gly (3.6 mg L-1), Rd (10 mg L-1), and co-exposures (Gly + NPs and Rd + NPs), or only to saline for 1, 6, and 12 h. Cell viability was assessed by Trypan blue, MTT, and neutral red assays. The generation of reactive oxygen species and total antioxidant capacity were also determined, while genotoxicity was quantified by the comet assay. Both NPs and Rd in isolation produced cytotoxic effects at 6 h and genotoxic effects at 1 and 6 h. Rd + NPs resulted in synergistic effects, intensifying the toxicity. Cells exposed to Gly did not present toxic effects and Gly + NPs resulted in the suppression of toxic effects observed for NPs. The presence of other components in Roundup (R) seems to favor its toxicity compared to the active ingredient. In conclusion, according to the in vitro model, the concentrations used were not safe for the ZFL lineage. (AU)

FAPESP's process: 17/21004-5 - Agriculture, micro/nanotechnology and environment: from evaluation of the mechanisms of action to studies of transport and toxicity
Grantee:Leonardo Fernandes Fraceto
Support type: Research Projects - Thematic Grants