| Full text | |
| Author(s): |
Sant'Ana, Monielle
[1]
;
Souza, Helena R.
[2, 3]
;
Possebon, Lucas
[2, 3]
;
Cornelio, Marinonio L.
[4]
;
Riffo-Vasquez, Yanira
[5]
;
Girol, Ana Paula
[2, 3]
;
Oliani, Sonia M.
[3, 1]
Total Authors: 7
|
| Affiliation: | [1] Sao Paulo Fed Univ, Dept Morphol & Genet, UNIFESP, Sao Paulo, SP - Brazil
[2] Univ Ctr Padre Albino UNIFIPA, Catanduva, SP - Brazil
[3] Sao Paulo State Univ, Inst Biosci Humanities & Exact Sci IBILCE, Dept Biol, UNESP, Lab Immunomorphol, Sao Jose Do Rio Preto Campus, Sao Jose Do Rio Preto, SP - Brazil
[4] Sao Paulo State Univ, Inst Biosci Humanities & Exact Sci IBILCE, Dept Phys, UNESP, Sao Jose Do Rio Preto Campus, Sao Jose Do Rio Preto, SP - Brazil
[5] Kings Coll London, Sackler Inst Pulm Pharmacol, Inst Pharmaceut Sci, London - England
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Pulmonary Pharmacology & Therapeutics; v. 61, APR 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Chronic obstructive pulmonary disease (COPD) is related to smoking and anti-inflammatory therapy is indicated. Among the mediators with anti-inflammatory properties, we highlight piperlongumine (PL), an alkaloid/amide of Piper longum. Here we evaluated the PL administration on an experimental model of respiratory inflammation resulting from exposure to cigarette smoke. Male Balb/c mice were exposed to burning of 10 commercial cigarettes, 2x/day, for five weeks on specific equipment. PL efficacy was evaluated in control, exposed to smoke without treatment and PL treated (2.0 mg/kg, 3x/week) groups. Animals were weighed and plethysmographic analyses performed at the end of the exposure protocol. Inflammatory cells were evaluated in the bronchoalveolar lavage (BAL) and hemoglobin and glucose in the blood. Lung fragments were processed for histopathological studies and AnxA1, COX-2, NF-kB and neutrophil elastase expressions. Plethysmography revealed that PL maintained pulmonary frequency, volume and ventilation parameters similar to controls, with respiratory volume reduction compared to untreated animals. Final weight was reduced in both exposed groups. PL decreased hemoglobin concentration, attenuated the reduction of glucose levels and reduced influx of lymphocytes, neutrophils and macrophages in BAL. Histopathologically occured infiltration of inflammatory cells, increase of the interalveolar septa and intra-alveolar spaces in untreated animals. But, PL administration recovered lung tissues and, immunohistochemically, promoted increased expression of AnxA1 and reduction of COX-2, NF-kB and neutrophil elastase. Together the results indicate that PL attenuates systemic and pulmonary inflammatory changes, partially by modulating the expression the endogenous AnxA1, and may represent a promising therapy in preventing the inflammation induced by cigarette smoke. (AU) | |
| FAPESP's process: | 16/02012-4 - Evaluation of the immunomodulatory activity of annexin A1 protein in the regulation of inflammatory disorders of the gastrointestinal system: studies in vivo and in vitro experimental models |
| Grantee: | Sonia Maria Oliani |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 12/13041-4 - Evaluation of skin heterologous graft using acellular matrices obtained from porcine skin and annexin A1 action as anti-inflammatory / immunosuppressive agent. |
| Grantee: | Kallyne Kioko Oliveira Mimura |
| Support Opportunities: | Scholarships in Brazil - Doctorate |