The role of periodontal treatment associated with photodynamic therapy on the modulation of systemic inflammation in the experimental model of asthma and periodontitis

Author(s):Show less -

Murakami-Malaquias-da-Silva, Felipe ^[1] ; Rosa, Ellen Perim ^[1] ; Oliveira, Jessica Gonzaga ^[1] ; Avelar, Isabella Sena ^[1] ; Palma-Cruz, Marlon ^[1] ; Fernandes Silva, Joao Gabriel ^[1] ; Rigonato-Oliveira, Nicole Cristine ^[1] ; Bussadori, Sandra Kalil ^[1] ; Negreiros, Renata Matalon ^[1] ; Ligeiro-de-Oliveira, Ana Paula ^[1] ; Lino-dos-Santos-Franco, Adriana ^[1] ; Horliana, Anna Carolina ^[1]

Affiliation

^[1] Univ Nove de Julho UNINOVE, Post Grad Program Biophoton Appl Hlth Sci, Rua Vergueiro 239-245, BR-01504000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 29, MAR 2020.
• Web of Science Citations: 0

Abstract

Background: The association of Periodontitis (P) with several systemic diseases, among them asthma (A), has been previously studied. As periodontal treatment (TTO) associated with photodynamic therapy (PDT) is able to treat P, the aim of this study is to verify whether periodontitis exerts systemic effects on asthma, and whether TTO, associated or not with PDT, is capable of altering the systemic course of both pathologies. Methods: 64 male Balb/c mice were divided into 8 groups (n=8): Basal (B), P, P+ TTO, P+ TTO+ PDT, Asthma, A+ P, A+ P+ TTO, A+ P+ TTO+ PDT. After 43 days, all animals were euthanized. The total and differential leukocyte count in serum, platelet count, alveolar bronchial lavage cell count, femoral lavage cell count in addition to the reactivity of the trachea, lung edema and gingiva cytokines were analyzed. The frequency of inflammatory cells was assessed via flow cytometry. One-way ANOVA test was used, followed by the Student-Newman-Keuls post-test. Results: There was an increase in the number of blood circulating eosinophils in group A when compared to group B (p < 0.01); this characterized the asthma experimental model. P (p < 0.05) presented a lower amount of cytokine TNF-alpha in the gingiva when compared to the Asthma group. Apart from that, there was no statistical difference found for the other analyzed parameters. Conclusion: These data contributed to elucidate that P and A, associated or not with TTO and PDT, are not able to interfere with the systemic parameters of Balb/c mice. (AU)

• FAPESP's process: 15/20535-1 - Evaluation of periodontal treatment in bronchiectasic patients
• Grantee: Anna Carolina Ratto Tempestini Horliana
• Support Opportunities: Regular Research Grants