High concentrations of waste anesthetic gases induce genetic damage and inflammation in physicians exposed for three years: A cross-sectional study

Author(s):Show less -

Braz, Mariana G. ^{[1, 2]} ; Carvalho, Lorena I. M. ^{[1, 2]} ; Chen, Chung-Yen O. ^[2] ; Blumberg, Jeffrey B. ^[2] ; Souza, Katina M. ^[1] ; Arruda, Nayara M. ^[1] ; Filho, Daniel A. A. ^[1] ; Resende, Ludimila O. ^[1] ; Faria, Renata T. B. G. ^[1] ; Canario, Clara d'A ^[1] ; de Carvalho, Lidia R. ^[3] ; Correa, Camila R. ^[4] ; Braz, Jose Reinaldo C. ^[1] ; Braz, Leandro G. ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Med Sch, Dept Anesthesiol, GENOTOX Lab UNIPEX, Botucatu, SP - Brazil

^[2] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Antioxidants Res Lab, Boston, MA 02111 - USA

^[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Bioestat, Botucatu, SP - Brazil

^[4] Sao Paulo State Univ Unesp, Med Sch, Dept Pathol, Botucatu, SP - Brazil

• Document type: Journal article
• Source: INDOOR AIR; v. 30, n. 3, p. 512-520, MAY 2020.
• Web of Science Citations: 0

Abstract

This cross-sectional study analyzed the impact of occupational waste anesthetic gases on genetic material, oxidative stress, and inflammation status in young physicians exposed to inhalational anesthetics at the end of their medical residency. Concentrations of waste anesthetic gases were measured in the operating rooms to assess anesthetic pollution. The exposed group comprised individuals occupationally exposed to inhalational anesthetics, while the control group comprised individuals without anesthetic exposure. We quantified DNA damage; genetic instability (micronucleus-MN); protein, lipid, and DNA oxidation; antioxidant activities; and proinflammatory cytokine levels. Trace concentrations of anesthetics (isoflurane: 5.3 +/- 2.5 ppm, sevoflurane: 9.7 +/- 5.9 ppm, and nitrous oxide: 180 +/- 150 ppm) were above international recommended thresholds. Basal DNA damage and IL-17A were significantly higher in the exposed group {[}27 +/- 20 a.u. and 20.7(19.1;31.8) pg/mL, respectively] compared to the control group {[}17 +/- 11 a.u. and 19.0(18.9;19.5) pg/mL, respectively], and MN frequency was slightly increased in the exposed physicians (2.3-fold). No significant difference was observed regarding oxidative stress biomarkers. The findings highlight the genetic and inflammatory risks in young physicians exposed to inhalational agents in operating rooms lacking adequate scavenging systems. This potential health hazard can accompany these subjects throughout their professional lives and reinforces the need to reduce ambient air pollution and consequently, occupational exposure. (AU)

• FAPESP's process: 13/12120-0 - Effect of occupational exposure to waste anesthetic gases in the oxidative stress
• Grantee: Daniel Abdalla Added Filho
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 13/21130-0 - Correlation between concentration of waste anesthetic gases with oxidative stress and immune response in medical residents occupationally exposed
• Grantee: Mariana Gobbo Braz
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/06420-7 - Evauation of DNA damage in medical residents occupationally exposed to waste anesthetic gases
• Grantee: Nayara Micarelli de Arruda
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 13/18075-7 - Concentrations of waste anesthetic gases and evaluation of DNA damage and oxidative stress in recently exposed medical personnel
• Grantee: Mariana Gobbo Braz
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 14/08661-9 - Cytokine profile in newly professionals exposed to waste anesthetic gases
• Grantee: Ludimila Oliveira Resende
• Support Opportunities: Scholarships in Brazil - Scientific Initiation