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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Leptospira interrogans LIC10774 is a multifunctional surface protein that binds calcium and interacts with host components

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Passalia, Felipe Jose [1, 2] ; Carvalho, Eneas [3] ; Heinemann, Marcos Bryan [4] ; Vieira, Monica Larucci [5] ; Nascimento, Ana Lucia T. O. [1, 2]
Total Authors: 5
[1] Inst Butantan, Lab Desenvolvimento Vacinas, BR-05503900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Programa Posgrad Interunidades Biotecnol, BR-05508900 Sao Paulo - Brazil
[3] Inst Butantan, Lab Bacteriol, BR-05503900 Sao Paulo - Brazil
[4] Univ Sao Paulo, Fac Med Vet & Zootecnia, Lab Zoonoses Bacterianas, Sao Paulo - Brazil
[5] Univ Fed Minas Gerais UFMG, ICB, Dept Microbiol, Belo Horizonte, MG - Brazil
Total Affiliations: 5
Document type: Journal article
Web of Science Citations: 0

Leptospirosis is a global re-emerging zoonosis, caused by pathogenic bacteria of the genus Leptospira. Humans are infected mainly through contact with contaminated water or soil. The understanding of the molecular mechanisms of leptospirosis through the characterization of unknown outer membrane proteins may contribute to the development of new treatments, diagnostic methods and vaccines. We have identified using bioinformatics analysis a protein that is encoded by the gene LIC10774, predicted to be localized at the leptospiral outer membrane and exhibit beta-roll folding. Surface exposure was confirmed by flow cytometry, ELISA and immunofluorescence-based confocal microscopy. Through circular dichroism spectroscopy and hydrophobic dye binding we have shown that rLIC10774 binds calcium ions, which imposes changes to secondary and tertiary structures. The recombinant protein was capable of binding to several host extracellular matrix and serum components. Therefore, we describe LIC10774 as a calcium-binding protein exposed in the outer surface of pathogenic leptospires with possible multifunctional roles in adhesion to host tissues, evasion of the immune system and participation in dissemination processes during leptospirosis. In addition, we hypothesize that the calcium binding is important for temperature-dependent functional roles during leptospirosis. (AU)

FAPESP's process: 17/00236-5 - Platelets at the interface of immunity, inflammation and hemostasis modulation in leptospirosis
Grantee:Mônica Larucci Vieira
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 14/50981-0 - Search for surface proteins among the genome sequences of Leptospira interrogans: functional and immunological characterization to understanding mechanisms involved in the bacterial pathogenesis
Grantee:Ana Lucia Tabet Oller Do Nascimento
Support type: Research Projects - Thematic Grants
FAPESP's process: 17/01102-2 - Biochemical and immunological evaluation of hypothetical surface proteins of Leptospira interrogans
Grantee:Felipe José Passalia
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 18/07054-2 - Platelets at the interface of immunity, inflammation and hemostasis modulation in leptospirosis
Grantee:Mônica Larucci Vieira
Support type: Scholarships in Brazil - Young Researchers