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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Competition Between Phenothiazines and BH3 Peptide for the Binding Site of the Antiapoptotic BCL-2 Protein

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Author(s):
do Carmo, Aline Lagoeiro [1] ; Bettanin, Fernanda [2] ; Almeida, Michell Almeida [3] ; Pantaleao, Simone Queiroz [1] ; Rodrigues, Tiago [1] ; Homem-de-Mello, Paula [1] ; Honorio, Kathia Maria [2, 1]
Total Authors: 7
Affiliation:
[1] Fed Univ ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP - Brazil
[2] Univ Sao Paulo, Escola Artes Ciencias & Humanidades, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Quim Sao Carlos, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: FRONTIERS IN CHEMISTRY; v. 8, APR 3 2020.
Web of Science Citations: 0
Abstract

The study of proteins and mechanisms involved in the apoptosis and new knowledge about cancer's biology are essential for planning new drugs. Tumor cells develop several strategies to gain proliferative advantages, including molecular alterations to evade from apoptosis. Failures in apoptosis could contribute to cancer pathogenesis, since these defects can cause the accumulation of dividing cells and do not remove genetic variants that have malignant potential. The apoptosis mechanism is composed by proteins that are members of BCL-2 and cysteine-protease families. BH3-only peptides are the ``natural{''} intracellular ligands of BCL-2 family proteins. On the other hand, studies have proved that phenothiazine compounds influence the induction of cellular death. To understand the characteristics of phenothiazines and their effects on tumoral cells and organelles involved in the apoptosis, as well as evaluating their pharmacologic potential, we have carried out computational simulation with the purpose of relating the structures of the phenothiazines with their biological activity. Since the tridimensional (3D) structure of the target protein is known, we have employed the molecular docking approach to study the interactions between compounds and the protein's active site. Hereafter, the molecular dynamics technique was used to verify the temporal evolution of the BCL-2 complexes with phenothiazinic compounds and the BH3 peptide, the stability and the mobility of these molecules in the BCL-2 binding site. From these results, the calculation of binding free energy between the compounds and the biological target was carried out. Thus, it was possible to verify that thioridazine and trifluoperazine tend to increase the stability of the BCL-2 protein and can compete for the binding site with the BH3 peptide. (AU)

FAPESP's process: 06/00995-9 - Characterization of the antitumoral properties of excited and ground state phenothiazines by focusing their action on mitochondria, lysosomes and biological membranes
Grantee:Tiago Rodrigues
Support type: Regular Research Grants
FAPESP's process: 16/07367-5 - Investigation of Phenothiazine-Induced Cell Death Mechanisms In Tumor Cells: Changes in Gene Expression, Role of Bcl-2 Family Proteins, and ER Stress
Grantee:Tiago Rodrigues
Support type: Regular Research Grants
FAPESP's process: 17/23416-9 - Photosensitizers: from fundamental properties to biological applications
Grantee:Paula Homem-de-Mello
Support type: Regular Research Grants
FAPESP's process: 16/24524-7 - STRUCTURAL ANALYSIS AND MOLECULAR MODELING STUDIES FOR NATURAL AND SYNTHETIC LIGANTS RELATED TO NEGLECTED DISEASES
Grantee:Kathia Maria Honorio
Support type: Regular Research Grants
FAPESP's process: 17/10118-0 - Study and application of electrochemical technology for the analysis and degradation of endocrine interferents: materials, sensors, processes and scientific dissemination
Grantee:Marcos Roberto de Vasconcelos Lanza
Support type: Research Projects - Thematic Grants