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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A novel BSA immobilizing manner on modified titanium surface ameliorates osteoblast performance

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Author(s):
Gomes, O. P. [1] ; Feltran, G. S. [2] ; Ferreira, M. R. [2] ; Albano, C. S. [2, 1] ; Zambuzzi, W. F. [2] ; Lisboa-Filho, P. N. [1]
Total Authors: 6
Affiliation:
[1] Sao Paulo State Univ, Sch Sci, Dept Phys, UNESP, Bauru, SP - Brazil
[2] Sao Paulo State Univ, Inst Biosci Botucatu, Dept Chem & Biochem, UNESP, Botucatu, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 190, JUN 2020.
Web of Science Citations: 0
Abstract

Surface modification of medical and dental devices, to improve their biocorrosion resistance and biocompatibility, can be achieved with the multidisciplinary field of biomaterials. Nanostructured titanium dioxide (TiO2) has been employed as surface modifier of titanium-based biomaterials because it can prevent the failure of the devices due to wear mechanisms. Moreover, this oxide surface is mostly terminated by hydroxyl groups (-OH) that can be directly functionalized with biomolecules to improve the biocompatibility of these devices. We explored the influence of 3-aminopropyltrimethoxysilane (APTMS) molecules as spacers in bovine serum albumin (BSA) protein immobilization on the physically hydroxylated surfaces of ruffle phase TiO2 films grown by reactive Radio Frequency (RF) magnetron sputtering. X-ray Photoelectron Spectroscopy (XPS) was used to examine the adsorption of BSA and APTMS on the hydroxylated surface of TiO2 thin films. For biological tests, BSA was directly immobilized on the film surface and on the APTMS monolayer. Biological analysis found better osteoblast performance considering gene markers related to cell adhesion after interacting directly with the surface modified by the immobilization of BSA, especially on the surface where this protein was immobilized by APTMS. Additionally, we addressed the relevance of this biointerfaces on extracellular matrix remodeling by zymography analysis. Altogether, our data provides new insights about the cellular and molecular mechanisms covering the improved osteoblastic response of the proposed surface modification. (AU)

FAPESP's process: 16/22186-7 - Protein Interaction in TiO2 Thin Films Surfaces
Grantee:Orisson Ponce Gomes
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 14/22689-3 - Microvesicle/proteins-mediated paracrine signaling among bone and endothelial cells during bone development and regeneration
Grantee:Willian Fernando Zambuzzi
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 17/15035-5 - Adsorption of bisphosphonates and proteins on TiO2 surfaces for bone implants
Grantee:Paulo Noronha Lisboa Filho
Support Opportunities: Regular Research Grants
FAPESP's process: 13/07296-2 - CDMF - Center for the Development of Functional Materials
Grantee:Elson Longo da Silva
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 14/20471-0 - Functionalized metal oxides thin films for the study of protein adsorption
Grantee:Paulo Noronha Lisboa Filho
Support Opportunities: Regular Research Grants