Brazilian berry extract (Myrciaria jaboticaba): A promising therapy to minimize prostatic inflammation and oxidative stress

Author(s)

Lamas, Celina A. ^[1] ; Kido, Larissa A. ^{[1, 2]} ; Hermes, Tulio A. ^[3] ; Nogueira-Lima, Ellen ^[1] ; Minatel, Elaine ^[1] ; Collares-Buzato, Carla B. ^[4] ; Marostica Jr, Mario R. ; Cagnon, Valeria H. A. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Sch Food Engn, Dept Food & Nutr, Sao Paulo - Brazil

^[3] ABC Med Sch, Dept Morphol & Physiol, Santo Andre, SP - Brazil

^[4] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Sao Paulo - Brazil

• Document type: Journal article
• Source: PROSTATE; v. 80, n. 11 MAY 2020.
• Web of Science Citations: 0

Abstract

Background Brazilian berry is a fruit popularly known as ``Jaboticaba,{''} rich in bioactive compounds with antioxidant and anti-inflammatory properties. Senescence and overweight are increasing worldwide and are considered risk factors to prostatic pathogenesis mainly due to oxidative and inflammatory processes induction. Thus, this study aimed to evaluate the effect of two increasing doses of the patented jaboticaba peel extract (PJE) on oxidative-stress and inflammation in the prostate of aging or high-fat-fed aging mice. Methods PJE and/or high-fat diet (HFD) treatments started with 11-month-old mice and lasted 60 days. The levels or the immunoexpression of different inflammatory (nuclear factor kappa B {[}NF kappa B], CD3+, cyclooxygenase 2 {[}COX-2], toll-like receptor 4 {[}TLR4], phosphorylated signal transducers and activators of transcription 3 {[}pSTAT-3], tumor necrosis factor alpha {[}TNF-alpha], interleukin 6 {[}IL-6], and IL-1 beta) and oxidative-stress (catalase, superoxide dismutase 2 {[}SOD2], glutathione reductase {[}GSR], reduced glutathione, and glutathione peroxidase 3 {[}GPx3]) related molecules were analyzed by western-blotting, immunohistochemistry, and enzyme-linked immunosorbent assays. Results Both PJE doses reduced the levels of oxidative-stress-related molecules (GPx3, GSR, catalase), lipid peroxidation (4-hydroxynonenal), inflammatory mediators (COX-2, TNF-alpha, and pSTAT-3) and CD3+ T cells number, which were associated with the maintenance of the glandular morphological integrity in aging and HFD-fed-aging mice. Nevertheless, only the high PJE dose reduced the NF kappa B and TLR4 levels in aging mice; and SOD2, IL-6, and IL-1 beta levels in HFD-aging mice. Aging itself promoted an oxidative inflammation in the prostate, interfering in the levels of the different oxidative-stress, lipid peroxidation, and inflammatory mediators evaluated, in association with high incidence of prostate epithelial and stromal damages. The HFD intake intensified aging alterations, showing an unfavorable prostatic microenvironment prone to oxidative and inflammatory damages. Conclusions PJE exerted a dose-dependent effect controlling inflammation and oxidative-stress in aging and HFD-fed aging mice prostate. This fact contributed to prostate microenvironment balance recovery, preserving the tissue architecture of this gland. Thus, the PJE emerges as a potential therapy to prevent inflammation and oxidative stress in the prostate. (AU)

• FAPESP's process: 18/11069-5 - Action of Brazilian berries on metabolic parameters and cognitive performance of obese animals
• Grantee: Mário Roberto Maróstica Junior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/50333-1 - COBRA: a novel compound discovery pipeline for rapid and cost-effective identification of bioactives with health beneficial effects in Brazilian crops
• Grantee: Glaucia Maria Pastore
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 15/25714-1 - Effects of jaboticaba peel extract therapy on the prostate biology and metabolic parameters of the mice FVB in relation to aging and hyperlipidic diet
• Grantee: Valéria Helena Alves Cagnon Quitete
• Support Opportunities: Regular Research Grants