| Full text | |
| Author(s): |
de Melo, Gabriela Dalmaso
[1]
;
Mello, Barbara Piffero
[1]
;
Ferreira, Catia Aparecida
[1]
;
Souto Godoy Filho, Carlos Alberto
[1]
;
Rocha, Cecilia Constantino
[1]
;
Silva, Amanda Guimaraes
[1]
;
Reese, Sydney Taylor
[2]
;
Madureira, Ed Hoffmann
[1]
;
Pohler, Ky Garrett
[2]
;
Pugliesi, Guilherme
[1]
Total Authors: 10
|
| Affiliation: | [1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Pirassununga, SP - Brazil
[2] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 - USA
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Theriogenology; v. 152, p. 94-105, AUG 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
We aimed to evaluate the accuracy of Interferon-tau stimulated genes (ISG) abundance in peripheral blood polymorphonuclear cells (PMNs) on D20 after fixed-time artificial insemination (FTAI; D0) as a pregnancy diagnosis method against CL evaluation by Doppler ultrasonography and progesterone (P4) concentrations on D20, as well as Pregnancy Associated Glycoproteins (PAG) concentrations on D25. Additionally, we evaluated the potential of ISG abundance in PMNs as pregnancy loss predictors. Nelore heifers (n = 103) and cows (n = 144) underwent estrous synchronization and were artificially inseminated on D0. Pregnancy was diagnosed by B-mode ultrasonography on D30 and D70, and after the final diagnosis, females were classified in four groups: Pregnant; Non-pregnant; Functional CL on D20 but non-pregnant (CL-NP) and Pregnancy loss between D30 and D70 (PL). After determining cutoff values, the Sensitivity (SE), Specificity (SP), Positive Predictive Value (PPV), Negative Predictive Value (NPV) and Accuracy (ACC) were determined for each method. All methods were classified as significant (P < 0.05) predictors of pregnancy. Both ISG expression and PAG concentrations were greater (P < 0.05) in pregnant females than in non-pregnant and CL-NP females but did not differ (P > 0.05) from the PL group. ISG15 expression was greater (P < 0.05) in heifers than in cows, but this difference was not found in OAS1 expreyyy (AU) | |
| FAPESP's process: | 17/13472-9 - Early pregnancy diagnosis in cattle through the expression of interferon-tau stimulated genes in neutrophils |
| Grantee: | Gabriela Dalmaso de Melo |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 18/20108-4 - Use of interferon-tau stimulated genes and bovine pregnancy-associated glycoproteins to predict pregnancy loss in Nelore cows and heifers |
| Grantee: | Gabriela Dalmaso de Melo |
| Support Opportunities: | Scholarships abroad - Research Internship - Master's degree |
| FAPESP's process: | 15/10606-9 - Innovations in the early pregnancy diagnosis in cattle |
| Grantee: | Guilherme Pugliesi |
| Support Opportunities: | Research Grants - Young Investigators Grants |