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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Use of whole-body cryosectioning and desorption electrospray ionization mass spectrometry imaging to visualize alkaloid distribution in poison frogs

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Author(s):
Jeckel, Adriana M. [1] ; Matsumura, Kunihiro [2] ; Nishikawa, Keisuke [2] ; Morimoto, Yoshiki [2] ; Saporito, Ralph A. [3] ; Grant, Taran [1] ; Ifa, Demian R. [4]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Biosci, Dept Zool, Sao Paulo - Brazil
[2] Osaka City Univ, Grad Sch Sci, Dept Chem, Sumiyoshi Ku, Osaka 5588585 - Japan
[3] John Carroll Univ, Dept Biol, Cleveland, OH 44118 - USA
[4] York Univ, Dept Chem, Ctr Res Mass Spectrometry, Toronto, ON - Canada
Total Affiliations: 4
Document type: Journal article
Source: Journal of Mass Spectrometry; v. 55, n. 6 JUN 2020.
Web of Science Citations: 0
Abstract

Ambient mass spectrometry is useful for analyzing compounds that would be affected by other chemical procedures. Poison frogs are known to sequester alkaloids from their diet, but the sequestration pathway is unknown. Here, we describe methods for whole-body cryosectioning of frogs and use desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to map the orally administered alkaloid histrionicotoxin 235A in a whole-body section of the poison frog Dendrobates tinctorius. Our results show that whole-body cryosectioning coupled with histochemical staining and DESI-MSI is an effective technique to visualize alkaloid distribution and help elucidate the mechanisms involved in alkaloid sequestration in poison frogs. (AU)

FAPESP's process: 16/09999-9 - Sequestration efficiency, metabolism and excretion of alkaloids in poison frogs and their non-sequestering relatives
Grantee:Adriana Moriguchi Jeckel
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 18/03577-0 - Mapping of Alkaloids in Dendrobatid Poison Frogs by Desorption Electrospray Ionization Mass Spectrometry Imaging (DESI-MSI)
Grantee:Adriana Moriguchi Jeckel
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 18/15425-0 - A multi-disciplinary approach to the study of amphibian diversification: phase 2
Grantee:Taran Grant
Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
FAPESP's process: 12/10000-5 - A multi-disciplinary approach to the study of amphibian diversification
Grantee:Taran Grant
Support Opportunities: Research Grants - Young Investigators Grants