Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Inhibitory Effect of a KSL-W Peptide-Loaded Poloxamer 407-Based Microemulsions for Buccal Delivery on Fusobacterium nucleatum Biofilm

Full text
Author(s):
Bernegossi, Jessica [1] ; Fontana, Carla Raquel [2] ; Caiaffa, Karina Sampaio [3] ; Duque, Cristiane [4] ; Chorilli, Marlus [1]
Total Authors: 5
Affiliation:
[1] Sao Paulo State Univ, Dept Drugs & Med, Lab Pharmaceut Technol, Sch Pharmaceut Sci, Univ Estadual Paulista, BR-14800903 Araraquara, SP - Brazil
[2] Sao Paulo State Univ, Dept Clin Anal, Lab Clin Microbiol, Sch Pharmaceut Sci, Univ Estadual Paulista, BR-14800903 Araraquara, SP - Brazil
[3] Sao Paulo State Univ, Aracatuba Dent Sch, Dept Prevent & Restorat Dent, Univ Estadual Paulista, BR-16015050 Sao Paulo - Brazil
[4] Sao Paulo State Univ, Aracatuba Dent Sch, Dept Pediat Dent & Publ Hlth, Univ Estadual Paulista, BR-16015050 Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF BIOMEDICAL NANOTECHNOLOGY; v. 16, n. 3, p. 390-397, MAR 2020.
Web of Science Citations: 0
Abstract

KSL-W peptide has demonstrated antibacterial and antifungal activity and inhibitory effects against oral biofilm. This study aimed to check out the effect of chlorhexidine (CLX) or KSL-W peptide-loaded poloxamer 407-based microemulsions for buccal delivery on Fusobacterium nucleatum (F. nucleatum) biofilm. The formulation (F) containing 10% copolymer poloxamer 407 dispersion (1%), 40% oleic acid and 50% PPG-5-CETETH-20 was characterized by polarized light microscopy (PLM), small-angle X-ray scattering (SAXS), rheology, bioadhesive and syringeability; and in the treatment of a biofilm produced by F. nucleatum. The darkfield images obtained by PLM and the SAXS curves with an extended peak indicated that the system was characteristic of microemulsions. In a continuous analysis, microemulsions exhibited Newtonian behavior. In frequency, the oscillatory analysis profile presented predominantly viscous behavior. Bioadhesive force detected in the analysis of F (7.4 +/- 1.81 mN.s) and syringeability (17.83 +/- 5.97 N.mm) being adequate values for buccal administration. After 4 h, KSL-W-loaded F shown over 20% higher effectiveness than chlorhexidine-loaded microemulsions. In conclusion, the KSL-W-loaded microemulsions showed a considerable reduction in F. nucleatum biofilm formation and presented promising structural properties for buccal drug delivery. (AU)

FAPESP's process: 14/22045-9 - Mucoadhesive precursor systems of liquids crystals for buccal administration of curcumin associated with photodynamic therapy in the treatment of oral candidiasis
Grantee:Jéssica Bernegossi
Support type: Scholarships in Brazil - Doctorate