Oxytocin and bone quality in the femoral neck of rats in periestropause

Author(s):Show less -

Fernandes, Fernanda ^[1] ; Stringhetta-Garcia, Camila Tami ^{[1, 2]} ; Peres-Ueno, Melise Jacon ^{[1, 2]} ; Fernandes, Fabiana ^{[1, 2]} ; de Nicola, Angela Cristina ^{[1, 2]} ; Castoldi, Robson Chacon ^[3] ; Ozaki, Guilherme ^[3] ; Quirino Louzada, Mario Jefferson ^{[1, 2]} ; Chaves-Neto, Antonio Hernandes ^{[4, 1, 2]} ; Ervolino, Edilson ^{[4, 1]} ; Menegati Dornelles, Rita Cassia ^{[4, 1, 2]}

Affiliation

^[1] Sao Paulo State Univ UNESP, SBFis, Programa Multictr Posgrad Ciencias Fisiol, Aracatuba, SP - Brazil

^[2] Menegati Dornelles, Rita Cassia, Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Aracatuba, SP, Brazil.Fernandes, Fernanda, Sao Paulo State Univ UNESP, SBFis, Programa Multictr Posgrad Ciencias Fisiol, Aracatuba, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Dept Physiotherapy, Presidente Prudente, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Aracatuba, SP - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 10, n. 1 MAY 13 2020.
• Web of Science Citations: 0

Abstract

The objective of this study is to identify whether oxytocin (OT) contributes to the reduction of osteopenia in the femoral neck of rats in periestropause. Animals in irregular estrous cycles received two NaCl injections (0.15 mol/L) or OT (134 mu g/kg) over a 12-h interval, and after thirty-five days without treatments, the biological sample collection was performed. The oxytocin group (Ot) demonstrated the highest enzymatic activity of alkaline phosphatase (p = 0.0138), lowest enzymatic activity of tartrate-resistant acid phosphatase (p = 0.0045), higher percentage of compact bone (p = 0.0359), cortical expression of runt-related transcription factor 2 (p = 0.0101), osterix (p = 0.0101), bone morphogenetic protein-2/4 (p = 0.0101) and periostin (p = 0.0455). Furthermore, the mineral-to-matrix ratio (nu 1PO4/Proline) was higher and type-B carbonate substitution (CO3/nu 1PO4) was lower (p = 0.0008 and 0.0303) in Ot group. The Ot showed higher areal bone mineral density (p = 0.0050), cortical bone area (p = 0.0416), polar moment of inertia, maximum, minimum (p = 0.0480, 0.0480, 0.0035), bone volume fraction (p = 0.0166), connectivity density (p < 0.0001), maximal load (p = 0.0003) and bone stiffness (p = 0.0145). In Ot percentage of cortical pores (p = 0.0102) and trabecular number (p = 0.0088) was lower. The results evidence action of OT in the reduction of osteopenia, suggesting that it is a promising anabolic strategy for the prevention of primary osteoporosis during the periestropause period. (AU)

• FAPESP's process: 10/09393-7 - Influence of resistant training and raloxifene on bone metabolism of ovariectomized and intact rats
• Grantee: Rita Cássia Menegati Dornelles
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/14262-7 - Nanostructured films from biologically-relevant materials
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/26779-4 - Effect of zoledronate during the alveolar healing process in rats: study involving the major risk factors for developing osteonecrosis of the jaws and assessment of antimicrobial photodynamic therapy (aPDT) as preventive therapy
• Grantee: Edilson Ervolino
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/14464-6 - Activity of the locus coeruleus noradrenergic neurons and the release of GnRH in acyclic female rats
• Grantee: Rita Cássia Menegati Dornelles
• Support Opportunities: Regular Research Grants