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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Outcome of blue, green, red, and white light on Metarhizium robertsii during mycelial growth on conidial stress tolerance and gene expression

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Author(s):
Dias, Luciana P. [1] ; Pedrini, Nicolas [2] ; Braga, Gilberto U. L. [3] ; Ferreira, Paulo C. [4] ; Pupin, Breno [4] ; Araujo, Claudineia A. S. [4] ; Corrochano, Luis M. [5] ; Rangel, Drauzio E. N. [6, 7]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo EEL USP, Escola Engn Lorena, BR-12602810 Lorena, SP - Brazil
[2] Univ Nacl La Plata, CONICET, CCT La Plata, Inst Invest Bioquim La Plata, Fac Ciencias Med, RA-1900 La Plata - Argentina
[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[4] Univ Vale Paraiba, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[5] Univ Seville, Fac Biol, Dept Genet, Ave Reina Mercedes 6, Apartado 1095, Seville 41080 - Spain
[6] Univ Brasil, BR-08230030 Sao Paulo, SP - Brazil
[7] Univ Brasil, BR-08230030 Sao Paulo, SP, Brazil.Dias, Luciana P., Univ Sao Paulo EEL USP, Escola Engn Lorena, BR-12602810 Lorena, SP - Brazil
Total Affiliations: 7
Document type: Journal article
Source: FUNGAL BIOLOGY; v. 124, n. 5, p. 263-272, MAY 2020.
Web of Science Citations: 4
Abstract

Fungi sense light and utilize it as a source of environmental information to prepare against many stressful conditions in nature. In this study, Metarhizium robertsii was grown on: 1) potato dextrose agar medium (PDA) in the dark (control); 2) under nutritive stress in the dark; and 3) PDA under continuous (A) white light; (B) blue light lower irradiance = LI; (C) blue light higher irradiance = HI; (D) green light; and (E) red light. Conidia produced under these treatments were tested against osmotic stress and UV radiation. In addition, a suite of genes usually involved in different stress responses were selected to study their expression patterns. Conidia produced under nutritive stress in the dark were the most tolerant to both osmotic stress and UV radiation, and the majority of their stress- and virulence-related genes were up-regulated. For osmotic stress tolerance, conidia produced under white, blue LI, and blue HI lights were the second most tolerant, followed by conidia produced under green light. Conidia produced under red light were the least tolerant to osmotic stress and less tolerant than conidia produced on PDA medium in the dark. For UV tolerance, conidia produced under blue light LI were the second most tolerant to UV radiation, followed by the UV tolerances of conidia produced under white light. Conidia produced under blue HI, green, and red lights were the least UV tolerant and less tolerant than conidia produced in the dark. The superoxide dismutases (sod1 and sod2), photolyases (6-4phr and CPDphr), trehalose-phosphate synthase (tps), and protease (pr1) genes were highly up-regulated under white light condition, suggesting a potential role of these proteins in stress protection as well as virulence after fungal exposure to visible spectrum components. (C) 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 12/15204-8 - Mechanistic study of the photodynamic inactivation of human and plant pathogenic fungi
Grantee:Gilberto Úbida Leite Braga
Support type: Regular Research Grants
FAPESP's process: 13/50518-6 - Stress related genes are induced by visible light during mycelial growth resulting in increased conidial tolerance to stress conditions
Grantee:Drauzio Eduardo Naretto Rangel
Support type: Regular Research Grants
FAPESP's process: 13/25964-2 - Visible light during mycelial growth alters gene expression and improves stress tolerance in entomopathogenic fungi
Grantee:Luciana Pereira Dias
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 18/20571-6 - International Symposium on Fungal Stress - ISFUS
Grantee:Drauzio Eduardo Naretto Rangel
Support type: Research Grants - Organization of Scientific Meeting
FAPESP's process: 10/06374-1 - Visible light during growth enhances conidial tolerance to different stress conditions in fungi
Grantee:Drauzio Eduardo Naretto Rangel
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 16/11386-5 - Mechanistic study of perception and response to visible light in the entomopathogenic fungus Metarhizium acridum
Grantee:Gilberto Úbida Leite Braga
Support type: Regular Research Grants