| Full text | |
| Author(s): |
Reis-de-Oliveira, G.
[1]
;
Zuccoli, G. S.
[1, 2]
;
Fioramonte, M.
[1, 2]
;
Schimitt, A.
[3, 4]
;
Falkai, P.
[4]
;
Almeida, V
[1, 2]
;
Martins-de-Souza, D.
[5, 1, 6, 7, 2]
Total Authors: 7
|
| Affiliation: | [1] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Lab Neuroprote, UNICAMP, Campinas, SP - Brazil
[2] Schimitt, A., Univ Sao Paulo, Inst Psychiat, Lab Neurosci LIM 27, Sao Paulo, Brazil.Reis-de-Oliveira, G., Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Lab Neuroprote, UNICAMP, Campinas, SP - Brazil
[3] Univ Sao Paulo, Inst Psychiat, Lab Neurosci LIM 27, Sao Paulo - Brazil
[4] Ludwig Maximilian Univ LMU, Dept Psychiat & Psychotherapy, Munich - Germany
[5] Conselho Nacl Desenvolvimento Cient & Tecnol, Inst Nacl Biomarcadores Neuropsiquiatria INBION, Sao Paulo - Brazil
[6] DOr Inst Res & Educ IDOR, Sao Paulo - Brazil
[7] Univ Estadual Campinas, Expt Med Res Cluster EMRC, Campinas, SP - Brazil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | JOURNAL OF PROTEOMICS; v. 223, JUL 15 2020. |
| Web of Science Citations: | 1 |
| Abstract | |
Schizophrenia is a psychiatric disorder that affects 21 million people worldwide. Despite several studies having been shown that some brain regions may play a critical role in the pathophysiology o f schizophrenia, the mo lecular basis to explain this diversity is still lacking. The cerebellum (CER), caudate nucleus (CAU), and posterior cingulate cortex (PCC) are areas associated with negative and cognitive symptoms in schizophrenia. In this study, w e performed shotgun proteomics o f the aforementioned brain regions, collected postmortem from pa tients with schizophrenia and compared with the mentally healthy group. In addition, w e performed a proteomic analysis o f nuclear and mitochondrial fractions of these same regions. Our results presented 106, 727 and 135 differentially regulated proteins in the CAU, PCC, and CER, respectively. Pathway enrichment analysis revealed dysfunctions associated with synaptic processes in the CAU, transport in the CER, and in energy metabolism in the PCC. In all brain areas, w e found that proteins related to oligodendrocytes and the metabolic processes were dysregulated in schizophrenia. Significance: Schizophrenia is a complex and heterogeneous psychiatric disorder. Despite much research having been done to increase the knowledge about the role o f each region in the pathophysiology o f this disorder, the molecular mechanisms underlying it are still lacking. We performed shotgun proteomics in the postmortem cerebellum (CER), caudate nucleus (CAU) and posterior cingulate cortex (PCC) from patients with schizophrenia and compared with healthy controls. Our findings suggest that each aforementioned region presents dysregulations in specific molecular pathways, such as energy metabolism in the PCC, transport in the CER, and synaptic process in the CAU. Additionally, these areas presented dysfunctions in oligodendrocytes and metabolic processes. Our results may highlight future directions for the development of novel clinical approaches for specific therapeutic targets. (AU) | |
| FAPESP's process: | 17/25588-1 - From the basic understanding to clinical biomarkers to schizophrenia: a neuroproteomics-centered multidisciplinary study |
| Grantee: | Daniel Martins-de-Souza |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 18/14666-4 - Influence of the endocannabinoid system on the energetic profile of neural cells during neurodevelopment: implications in Schizophrenia |
| Grantee: | Giuliana da Silva Zuccoli |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 18/03673-0 - Biochemical effects of cannabinoids on oligodendrocytes: implications for schizophrenia |
| Grantee: | Daniel Martins-de-Souza |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/18242-1 - Biochemical pathways affected by cannabinoid drugs of human oligodendrocytes |
| Grantee: | Valéria de Almeida |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 19/00098-7 - Multi-User Equipment approved in grant 2017/25588-1: cromatógrafo Acquity UPLC I-Class |
| Grantee: | Daniel Martins-de-Souza |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 16/18715-4 - Establishment of the hnRNPs role on schizophrenia through protein interaction network mapping |
| Grantee: | Mariana Fioramonte |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/01410-1 - Evaluating the effects of clozapine on immune system cells |
| Grantee: | Guilherme Reis-De-Oliveira |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 14/10068-4 - Multi-User Equipment approved in grant 13/08711-3: mass spectrometer waters SYNAPT G2-Si HDMS + nanoACQUITY UPLC |
| Grantee: | Daniel Martins-de-Souza |
| Support Opportunities: | Multi-user Equipment Program |