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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mycobacterium tuberculosis-infected alveolar epithelial cells modulate dendritic cell function through the HIF-1 alpha-NOS2 axis

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Author(s):
Rodrigues, Tamara Silva [1] ; Alvarez, Annie Rocio Pineros [2] ; Gembre, Ana Flavia [1, 3] ; Forni, Maria Fernanda Pereira de Araujo Demonte [1, 4] ; de Melo, Bruno Marcel Silva [5] ; Alves Filho, Jose Carlos Farias [5] ; Camara, Niels Olsen Saraiva [4] ; Bonato, Vania Luiza Deperon [1, 3]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, Ribeirao Preto - Brazil
[2] Indiana Univ Purdue Univ, Indianapolis, IN 46202 - USA
[3] Camara, Niels Olsen Saraiva, Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Transplantat Immunol Lab, Sao Paulo, Brazil.Rodrigues, Tamara Silva, Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, Ribeirao Preto - Brazil
[4] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Transplantat Immunol Lab, Sao Paulo - Brazil
[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Ribeirao Preto - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Journal of Leukocyte Biology; JUN 2020.
Web of Science Citations: 0
Abstract

Tuberculosis kills more than 1 million people every year, and its control depends on the effective mechanisms of innate immunity, with or without induction of adaptive immune response. We investigated the interaction of type II alveolar epithelial cells (AEC-II) infected byMycobacterium tuberculosiswith dendritic cells (DCs). We hypothesized that the microenvironment generated by this interaction is critical for the early innate response against mycobacteria. We found that AEC-II infected byM. tuberculosisinduced DC maturation, which was negatively regulated by HIF-1 alpha-inducible NOS2 axis, and switched DC metabolism from an early and short peak of glycolysis to a low energetic status. However, the infection of DCs byM. tuberculosisup-regulated NOS2 expression and inhibited AEC-II-induced DC maturation. Our study demonstrated, for the first time, that HIF-1 alpha-NOS2 axis plays a negative role in the maturation of DCs duringM. tuberculosisinfection. Such modulation might be useful for the exploitation of molecular targets to develop new therapeutic strategies against tuberculosis. (AU)

FAPESP's process: 18/08479-7 - Multi-user equipment approved in grant 17/05264-7: analisador de fluxo extracelular Seahorse XFe96
Grantee:Niels Olsen Saraiva Câmara
Support type: Multi-user Equipment Program
FAPESP's process: 17/16538-0 - The role of HIF-1a in the interaction of type II alveolar epithelial cells and dendritic cells during the immune response against Mycobacterium tuberculosis
Grantee:Tamara Silva Rodrigues
Support type: Scholarships in Brazil - Master
FAPESP's process: 17/21629-5 - Strategies to study pulmonary inflammation during comorbidities
Grantee:Vânia Luiza Deperon Bonato
Support type: Research Projects - Thematic Grants