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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Circulating RNA Transcriptome of Pregnant Women with TSH Just Above the Trimester-Specific Reference and its Correlation with the Hypertensive Phenotype

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Author(s):
de Lima Hirata, Andrea Harumy [1] ; de Jesus Rocha, Luiz Antonio [1, 2] ; da Silva, Valdelena Alessandra [1, 2] ; de Almeida, Robson Jose [1, 2] ; Bacigalupo, Lucas dos Santos [1, 2, 3] ; Varela, Patricia [1, 4] ; Martins, Leonardo [1, 4] ; Pesquero, Joao Bosco [4] ; Delle, Humberto [1, 2] ; Camacho, Cleber Pinto [1, 2]
Total Authors: 10
Affiliation:
[1] Univ Nove Julho Uninove, Postgrad Program Med, Mol Innovat & Biotechnol Lab, Sao Paulo, SP - Brazil
[2] Pesquero, Joao Bosco, Univ Fed Sao Paulo UNIFESP, Ctr Res \& Mol Diagnost Genet Dis, Dept Biophys, Sao Paulo, SP, Brazil.de Lima Hirata, Andrea Harumy, Univ Nove Julho Uninove, Postgrad Program Med, Mol Innovat & Biotechnol Lab, Sao Paulo, SP - Brazil
[3] Conjunto Hosp Mandaqui, Dept Obstet & Gynecol, Sao Paulo, SP - Brazil
[4] Univ Fed Sao Paulo UNIFESP, Ctr Res & Mol Diagnost Genet Dis, Dept Biophys, Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 10, n. 1 APR 15 2020.
Web of Science Citations: 0
Abstract

During gestation, a woman's body undergoes physiological changes that alter thyroid function. Pregnant women with hypothyroidism may exhibit gestational complications, including hypertension and preeclampsia. We investigated differentially expressed genes (DEGs) in circulating RNAs from pregnant women with TSH levels just above the normal range to determine the impact of a mild elevation of TSH in pregnancy. We selected three women with healthy thyroid pregnancy (HTP), three pregnant women with gestational hypothyroidism (GHT), and three nonpregnant women (NPG) to construct transcriptome libraries. We also compared our results with data from the GEO dataset and DisGeNET. We identified 1500 DEG in GHT and 1656 DEG in HTP. From GEO dataset, we recognized 453 DEGs in trimester-specific plasma RNA, 1263 DEGs in placental tissues from healthy women, 1031 DEGs from preeclamptic uteroplacental tissues and 1657 DEGs from placental tissues from severely preeclamptic women. In this scenario, 12.26% and 12.86% genes were shared between these datasets in GHT and HTP, respectively. We stablished 62 genes in GHT DEGs related to hypertensive phenotype hallmarks. In conclusion, even in women with a mild TSH increment, we were able to detect some DEGs that could be associated with a hypertensive phenotype. (AU)

FAPESP's process: 14/04193-0 - Gene expression analysis of tissue health in subjects with clinical and subclinical thyroid dysfunction
Grantee:Cléber Pinto Camacho
Support type: Regular Research Grants