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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Polysaccharide-rich hydrogel formulation combined with photobiomodulation repairs UV-induced photodamage in mice skin

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Author(s):
Grosso Neves, Lia Mara [1] ; Parizotto, Nivaldo Antonio [2, 3, 4] ; Tim, Carla Roberta [2, 3] ; Floriano, Elaine Medeiros [5] ; Vianna Lopez, Renata F. [6] ; Venancio, Tiago [7] ; Fernandes, Joao Batista [7] ; Cominetti, Marcia Regina [1]
Total Authors: 8
Affiliation:
[1] Univ Fed Sao Carlos, Dept Gerontol, Lab Biol Aging LABEN, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos, Joint Grad Program Phys Therapy, Sao Carlos, SP - Brazil
[3] Univ Araraquara, Postgrad Program Biotechnol Regenerat Med & Med C, Araraquara, SP - Brazil
[4] Univ Brazil, Postgrad Program Biomed Engn, Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Dept Pathol & Legal Med, Ribeirao Preto, SP - Brazil
[6] Univ Sao Paulo, Ribeira Preto Sch Pharmaceut Sci FCFRP, Ribeirao Preto, SP - Brazil
[7] Univ Fed Sao Carlos, Dept Chem, Sao Carlos, SP - Brazil
Total Affiliations: 7
Document type: Journal article
Source: WOUND REPAIR AND REGENERATION; JUN 2020.
Web of Science Citations: 0
Abstract

Prolonged skin exposure to ultraviolet radiation (UVR) induces premature aging in both the epidermis and the dermis. Chronic exposure to UVR induces the activation of mitogen-activated protein kinase (MAPK) signaling pathway, activating c-Jun, c-Fos expression, and transcription factor of AP-1 activating protein. AP-1 activation results in the positive induction of matrix metalloproteinase (MMP) synthesis, which degrade skin collagen fibers. Polysaccharides from the fruit ofLycium barbarum(LBP fraction) have a range of activities and have been demonstrate to repair the photodamage. In different approaches, laser application aims to recover the aged skin without destroying the epidermis, promoting a modulation, called photobiomodulation (PBM), which leads to protein synthesis and cell proliferation, favoring tissue repair. Here we developed a topical hydrogel formulation from a polysaccharide-rich fraction ofLycium barbarumfruits (LBP). This formulation was associated with PBM (red laser) to evaluate whether the isolated and combined treatments would reduce the UVR-mediated photodamage in mice skin. Hairless mice were photoaged for 6 weeks and then treated singly or in combination with LBP and PBM. Histological, immunohistochemistry, and immunofluorescence analyses were used to investigate the levels of c-Fos, c-Jun, MMP-1, -2, and -9, collagen I, III, and FGF2. The combined regimen inhibited UVR-induced skin thickening, decreased the expression of c-Fos and c-Jun, as well as MMP-1, -2, and -9 and concomitantly increased the levels of collagen I, III, and FGF2. The PBM in combination with LBP treatment is a promising strategy for the repair of photodamaged skin, presenting potential clinical application in skin rejuvenation. (AU)

FAPESP's process: 13/27021-8 - Effects of the fruit extract of Lycium barbarum L. and red laser in the prevention and treatment of photoaging in hairless mice SKH:hr-1
Grantee:Lia Mara Grosso Neves
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/24940-8 - EFFECTIVENESS OF STRUCTURAL CHANGES IN [10]-GINGEROL MOLECULE IN COMBINATION WITH THE CHEMOTHERAPEUTIC DOXORUBICIN FOR THE TREATMENT OF BREAST CANCER: IN VITRO AND IN VIVO STUDIES. ABSTRACT
Grantee:Márcia Regina Cominetti
Support type: Regular Research Grants
FAPESP's process: 12/25299-6 - Integrated studies for leaf cutting control
Grantee:João Batista Fernandes
Support type: Research Projects - Thematic Grants