| Full text | |
| Author(s): |
Sibinelli-Sousa, Stephanie
[1]
;
Hespanhol, Julia T.
[1]
;
Nicastro, Gianlucca G.
[1]
;
Matsuyama, Bruno Y.
[2]
;
Mesnage, Stephane
[3]
;
Patel, Ankur
[3]
;
de Souza, Robson F.
[1]
;
Guzzo, Cristiane R.
[1]
;
Bayer-Santos, Ethel
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo - Brazil
[3] Univ Sheffield, Dept Mol Biol & Biotechnol, Sheffield S10 2TN, S Yorkshire - England
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | CELL REPORTS; v. 31, n. 12 JUN 23 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Type VI secretion systems (T6SSs) are nanomachines used by bacteria to inject toxic effectors into competitors. The identity and mechanism of many effectors remain unknown. We characterized a Salmonella T6SS antibacterial effector called TIde1 that is toxic in target-cell periplasm and is neutralized by its cognate immunity protein (TIdi1), Microscopy analysis reveals that cells expressing TIde1 stop dividing and lose cell envelope integrity. Bioinformatic analysis uncovers similarities between TIde1 and the catalytic domain of L,D-transpeptidases. Point mutations on conserved catalytic residues abrogate toxicity. Biochemical assays reveal that TIde1 displays both L,D-carboxypeptidase activity by cleaving peptidoglycan tetrapeptides between meso-diaminopimelic acid(3) and D-alanine(4) and L,D-transpeptidase exchange activity by replacing D-alanine(4) by a non-canonical D-amino acid. Phylogenetic analysis shows that TIde1 homologs constitute a family of T6SS-associated effectors broadly distributed among Proteobacteria. This work expands our current knowledge about bacterial effectors used in interbacterial competition and reveals a different mechanism of bacterial antagonism. (AU) | |
| FAPESP's process: | 18/04553-8 - Function of type VI secretion systems of pathogenic bacteria in the interaction with eukaryotic cells |
| Grantee: | Ethel Bayer Santos |
| Support Opportunities: | Scholarships in Brazil - Young Researchers |
| FAPESP's process: | 17/02178-2 - Function of type VI secretion systems of pathogenic bacteria in the interaction with eukaryotic cells |
| Grantee: | Ethel Bayer Santos |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 16/09047-8 - Comparative genomics of bacterial toxins associated with type IV secretion systems |
| Grantee: | Robson Francisco de Souza |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/00195-2 - Understanding the c-di-GMP signaling networks and the Type II secretion system present into the human pathogen Leptospira interrogans Copenhageni |
| Grantee: | Cristiane Rodrigues Guzzo Carvalho |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/17303-7 - Structure and function of bacterial secretion systems |
| Grantee: | Shaker Chuck Farah |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 18/25316-4 - Function of SPI-6 T6SS and SPI-19 T6SS of Salmonella during competition with the gut microbiota |
| Grantee: | Julia Takuno Hespanhol |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 16/00458-5 - Structural and functional characterization of Xanthomonas citri type IV secretion system. |
| Grantee: | Bruno Yasui Matsuyama |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 18/13819-1 - Analysis of the activation dynamics of the type 6 secretion system of Salmonella during infection of eukaryotic cells |
| Grantee: | Stephanie Sibinelli de Sousa |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |