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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

QTL and eQTL mapping associated with host response toCandidatusLiberibacter asiaticus in citrandarins

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Author(s):
Soratto, Tatiany Aparecida Teixeira [1, 2] ; Curtolo, Maiara [3, 1] ; Marengo, Samanta [4] ; Dezotti, Ana Lucia [1] ; Lima, Romulo Pedro Macedo [1, 5] ; Gazaffi, Rodrigo [6] ; Machado, Marcos Antonio [1] ; Cristofani-Yaly, Mariangela [1]
Total Authors: 8
Affiliation:
[1] Inst Agron Campinas, Ctr APTA Citros Sylvio Moreira, BR-13490970 Cordeiropolis, SP - Brazil
[2] Univ Fed Santa Catarina, Ctr Ciencias Biol, Lab Bioinformat, BR-88040970 Florianopolis, SC - Brazil
[3] Univ Estadual Campinas, BR-13083862 Campinas, SP - Brazil
[4] Hill Labs, Hamilton 3204 - New Zealand
[5] Univ Estado Sao Paulo, Inst Biociencias, Dept Genet, BR-18618000 Botucatu, SP - Brazil
[6] Univ Fed Sao Carlos, Ctr Ciencias Agr, BR-13600970 Araras, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: TROPICAL PLANT PATHOLOGY; v. 45, n. 6, SI MAY 2020.
Web of Science Citations: 0
Abstract

Huanglongbing (HLB) is a severe disease of citrus caused by the bacteriumCandidatusLiberibacter. In America, the most common species isCandidatusLiberibacter asiaticus (CLas). In a previous study of the Citrus-HLB pathosystem, our group found differences in CLas multiplication in a population of hybrids obtained by hybridization betweenCitrus sunkiHort. Ex Tan. andPoncirus trifoliata(L.) Raf. cv. Rubidoux. The bacterial concentration was higher inC. sunkithan inP. trifoliata. Thus, this study aims to map phenotypic (QTL) and gene expression (eQTL) data associated with host response to CLas in the linkage groups (LGs) of the previously constructed parental maps ofC. sunkiandP. trifoliatacv. Rubidoux. For the phenotypic analysis, we used a population of 79 F1 hybrids (termed citrandarins) betweenC. sunkiandP. trifoliata. CLas and starch were quantified in the leaves of the plants two years after pathogen inoculation, allowing the classification of hybrids as resistant, tolerant, and susceptible. The expression of 14 candidate genes was measured in 72 hybrids of the population and used as expression data for the eQTL mapping. We located nine QTL and 52 eQTL in theC. sunkimap and 17 QTL and 40 eQTL in theP. trifoliatamap. The overlap of eQTL of the majority of genes with QTL from the phenotypic data indicates that the genes are related to the phenotype and are probably related to pathogen infection. (AU)

FAPESP's process: 16/22133-0 - Genome editing aimed at QTLs validation associated with tolerance Candidatus Liberibacter asiaticus in citrandarins (Citrus sunki x Poncirus trifoliata)
Grantee:Maiara Curtolo
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/50880-0 - INCT 2014: comparative and functional genomics and citrus-assisted breeding
Grantee:Marcos Antonio Machado
Support type: Research Projects - Thematic Grants
FAPESP's process: 11/18605-0 - Obtaining and evaluation of new varieties of scion and rootstock for citrus
Grantee:Mariângela Cristofani-Yaly
Support type: Research Projects - Thematic Grants
FAPESP's process: 18/00133-4 - Trials with citrandarins as rootstock for the Pêra and Valencia sweet oranges and the Tahiti acid lime
Grantee:Mariângela Cristofani-Yaly
Support type: Regular Research Grants