Antibacterial and antifungal activity of crude and freeze-dried bacteriocin-like inhibitory substance produced by Pediococcus pentosaceus

Author(s)

de Souza de Azevedo, Pamela Oliveira ^[1] ; Nobrega Mendonca, Carlos Miguel ^[1] ; Ramos Moreno, Ana Carolina ^[2] ; Iank Bueno, Antonio Vinicius ^[3] ; Yokomizo de Almeida, Sonia Regina ^[4] ; Seibert, Liane ^[5] ; Converti, Attilio ^[6] ; Watanabe, Ii-Sei ^[4] ; Gierus, Martin ^[7] ; de Souza Oliveira, Ricardo Pinheiro ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Biochem & Pharmaceut Technol, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Biomed Sci Inst, Dept Microbiol, Sao Paulo, SP - Brazil

^[3] Univ Maringa, Dept Zootech, Maringa, PR - Brazil

^[4] Univ Sao Paulo, Biomed Sci Inst, Dept Anat, Sao Paulo - Brazil

^[5] Univ Fed Santa Maria, Dept Anim Sci, Lab Ecol & Nat Grassland, Santa Maria, RS - Brazil

^[6] Univ Genoa, Dept Civil Chem & Environm Engn, Pole Chem Engn, Via Opera 15, I-16145 Genoa - Italy

^[7] Univ Nat Resources & Life Sci BOKU, Inst Anim Nutr Livestock Prod & Nutr Physiol TTE, Dept Agrobiotechnol, IFA Tulln, Vienna - Austria

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 10, n. 1 JUL 23 2020.
• Web of Science Citations: 5

Abstract

Pediococcus pentosaceus LBM 18 has shown potential as producer of an antibacterial and antifungal bacteriocin-like inhibitory substance (BLIS). BLIS inhibited the growth of spoilage bacteria belonging to Lactobacillus, Enterococcus and Listeria genera with higher activity than Nisaplin used as control. It gave rise to inhibition halos with diameters from 9.70 to 20.00 mm, with Lactobacillus sakei being the most sensitive strain (13.50-20.00 mm). It also effectively suppressed the growth of fungi isolated from corn grain silage for up to 25 days and impaired morphology of colonies by likely affecting fungal membranes. These results point out that P. pentosaceus BLIS may be used as a new promising alternative to conventional antibacterial and antifungal substances, with potential applications in agriculture and food industry as a natural bio-controlling agent. Moreover, cytotoxicity and cell death induction tests demonstrated cytotoxicity and toxicity of BLIS to human colon adenocarcinoma Caco-2cells but not to peripheral blood mononuclear cells, with suggests possible applications of BLIS also in medical-pharmaceutical applications. (AU)

• FAPESP's process: 18/25511-1 - Bioprospecting of bacteriocin-producing bacteria: from culture optimization to the application in animal production systems
• Grantee: Ricardo Pinheiro de Souza Oliveira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/04385-8 - Construction of a pediocin-producing recombinant lineage from agroindustrial waste
• Grantee: Ricardo Pinheiro de Souza Oliveira
• Support Opportunities: Regular Research Grants